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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,520 results for: try

Showing: 7221 - 7240 of 9520 results

  1. HTR5-DuET

    Plasmid
    #213325
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR5 (HTR5A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213325-plasmid-map-sequence-id-430860

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  2. HTR7-DuET

    Plasmid
    #213327
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR7 (HTR7 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213327-plasmid-map-sequence-id-430861

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  3. HTR2B-DuET

    Plasmid
    #213322
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR2B (HTR2B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213322-plasmid-map-sequence-id-430858

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  4. HTR1A-DuET

    Plasmid
    #213316
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    HTR1A (HTR1A Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213316-plasmid-map-sequence-id-430853

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  5. F2RL3-DuET

    Plasmid
    #213237
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    F2RL3 (F2RL3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213237-plasmid-map-sequence-id-430795

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  6. F2RL1-DuET

    Plasmid
    #213235
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    F2RL1 (F2RL1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213235-plasmid-map-sequence-id-430793

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  7. pFSynW SYT9 D197, 199, 330, 332N IRES GFP

    Plasmid
    #195703
    Purpose
    Lentiviral plasmid encoding SYT9 with D197, 199, 330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter
    Depositor
    Edwin Chapman
    Article
    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.
    Insert
    Syt9 (Syt5 Mouse)
    Use
    Lentiviral
    Mutation
    full-length mouse SYT9 with D197, 199, 330, 332N …
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195703-plasmid-map-sequence-id-383663

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  14. pKTop::sptse5-CT (1169-1281)

    Plasmid
    #192950
    Purpose
    For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1281)
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1281)
    Available Since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192950-plasmid-map-sequence-id-380607

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  16. pKTop::sptse5-CT (1169-1300)

    Plasmid
    #192951
    Purpose
    For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1300)
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for sptse5 (1169-1300)
    Available Since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192951-plasmid-map-sequence-id-380604

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  18. KTK_219

    Plasmid
    #180534
    Purpose
    Entry vector containing K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)
    Expression
    Bacterial
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    180534-plasmid-map-sequence-id-369688

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  19. KTK_223

    Plasmid
    #180538
    Purpose
    Entry vector containing K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)
    Expression
    Bacterial
    Available Since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    180538-plasmid-map-sequence-id-369687

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  20. KTK_226

    Plasmid
    #180552
    Purpose
    Entry vector containing K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)
    Depositor
    Tom Ellis
    Article
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Insert
    K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)
    Expression
    Bacterial
    Available Since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    180552-plasmid-map-sequence-id-359396

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Showing: 7221 - 7240 of 9520 results