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pTol1-uas-mAKT-2a-nlstdTomato

Plasmid

#67706

Purpose

Tol1 expression plasmid with UAS-driven activated Akt-2A-myc tagged nlsTdTomato

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

mAKT-2A-nlstdTomato

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
LZF1723: mI12b-EGFP

Plasmid

#104123

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

mI12b

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PXWS80LBS320tetO

Plasmid

#160846

Purpose

LBS-tetO hybrid sponge with 80 repeats of LBS and 320 repeats of tetO

Depositor

Baojun Wang

Article

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(80 X LBS)-(320 X tetO)-t

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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MYC sgRNA5

Plasmid

#100557

Purpose

Expresses MYC sgRNA5. Target sequence GAGAGGCAGAGGGAGCGAGC

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

MYC sgRNA5

Promoter

U6

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMcSrc

Plasmid

#17672

Depositor

David Shalloway

Article

Johnson et al Mol Cell Biol. 1985 May . 5(5):1073-83.

Insert

c-src (SRC Chicken)

Expression

Mammalian

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pLNL1130

Plasmid

#160861

Purpose

PJ23110-RBS-AbWS/DGAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

O-acyltransferase WSD

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pSPK1

Plasmid

#18876

Depositor

Jay Keasling

Article

Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

Insert

polyphosphate kinase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only
p209 pCMV-cre-K

Plasmid

#8394

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

Modified 5' Cre

Use

Cre/Lox

Expression

Mammalian

Mutation

Modified 5' region of Cre

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pJJH1242

Plasmid

#36909

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Esf1

Plasmid

#99987

Purpose

Express yeast Esf1 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Esf1 (ESF1 Budding Yeast)

Tags

Y2H binding domain

Expression

Yeast

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MazF-K56A

Plasmid

#164274

Purpose

To express recombinant MazF K56A point mutant protein

Depositor

Guanzheng Luo

Article

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Insert

MazF

Expression

Bacterial

Mutation

K56A

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-T-protein

Plasmid

#12652

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2004 Nov 26. 279(48):50514-

Insert

T-protein

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pBN8

Plasmid

#86711

Purpose

Cloning plasmid for MosSCI transgenesis in C. elegans

Depositor

Peter Askjaer

Article

Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

Type

Empty backbone

Expression

Worm

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiN 5E6

Plasmid

#37449

Purpose

Lentiviral expression of HPV 5E6 in mammalian cells

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

HPV 5E6 (E6 HPV)

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pPERPlucFL

Plasmid

#14811

Depositor

Tyler Jacks

Article

Reczek et al Mol Cancer Res. 2003 Dec. 1(14):1048-57.

Insert

PERP (Perp Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pDEST17-hOPTN-D474N

Plasmid

#23055

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

Optineurin (OPTN Human)

Tags

His

Expression

Bacterial

Mutation

D474N

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pJJH1338

Plasmid

#36912

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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FuPick-shRNA

Plasmid

#31614

Depositor

Robert Malenka

Article

Citri et al J Neurosci. 2010 Dec 8. 30(49):16437-52.

Insert

PICK1 shRNA (Pick1 Rat)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM30D

Plasmid

#87084

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30D (Trim30d Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Vh-HA-3xDHFR

Plasmid

#20219

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Tags

HA and Vh

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only