We narrowed to 15,882 results for: LEA

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pT7-EGFP-C1-HsNot1iso1_1085-1605_K

Plasmid

#146807

Purpose

Mammalian Expression of HsNot1iso1_1085-1605

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1085-1605 (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM1-RRM2-V5His6_H

Plasmid

#146490

Purpose

Insect Expression of DmPABPC1-RRM1-RRM2

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM1-RRM2 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM2-RRM3-V5His6_H

Plasmid

#146491

Purpose

Insect Expression of DmPABPC1-RRM2-RRM3

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM2-RRM3 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-RRM3-RRM4-V5His6_H

Plasmid

#146492

Purpose

Insect Expression of DmPABPC1-RRM3-RRM4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM3-RRM4 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182-V5His6_H

Plasmid

#146499

Purpose

Insect Expression of DmGW182

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmGW182 (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmPABPC1-RRM1-RRM2-V5His6_H

Plasmid

#146479

Purpose

Insect Expression of DmPABPC1-RRM1-RRM2

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmPABPC1-RRM1-RRM2 (pAbp Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2_529-715

Plasmid

#196543

Purpose

Expression of myc-Eps8L2_529-715

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Tags

myc

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2

Plasmid

#196542

Purpose

Expression of myc-Eps8L2-FL

Depositor

Edgar Gomes

Article

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCav1 in pT3TS-Dest

Plasmid

#194293

Purpose

In vitro transcription of mKate2 tagged zebrafish caveolin1 from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone KZW

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin (cav1.S Frog)

Use

In vitro transcription of mrna

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1a

Plasmid

#194291

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1a with fusion tag at the N-terminus. Parton lab clone KXM

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 exon

Plasmid

#176033

Purpose

A vector for the CRISPR-Cas9 system targeting an exon of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-dTLN1 intron

Plasmid

#176224

Purpose

A vector for the CRISPR-Cas9 system targeting an intron of dog TLN1 (talin1)

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

A gRNA targeting the dog TLN1 gene and the cDNA of Cas9 (TLN1 )

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a

Plasmid

#182126

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12aen

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Plasmid

#182121

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Plasmid

#182124

Purpose

pET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.NWS_mCherry-NLS

Plasmid

#178282

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.NWS and Nuclear Localization Signal

Promoter

eF1a

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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HIS_TEV_hsSYX3

Plasmid

#179290

Purpose

E. coli expression plasmid (T7lac promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Insert

Syx

Tags

His6 + TEV protease cleavage site

Expression

Bacterial

Mutation

residues 393-792 from accession number NP_0010361…

Promoter

T7lac

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHcRed-NPMMLF1nes-C1

Plasmid

#131849

Purpose

Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Mutation

This is a control deletion contruct of the cytop…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Arg57Ala

Plasmid

#180759

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Arg57Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Arg57Ala, Leu1…

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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