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pYPQ141-ZmUbi-RZ-Cas12j2

Plasmid

#173926

Purpose

Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

Plasmid

#132686

Purpose

AAV plasmid encoding mouse ACY1 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Acylase I (Acy1, Acy1 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FCK-ss-Prl-Arch-GFP

Plasmid

#22224

Purpose

Lentiviral expression of Arch-GFP, with N-terminal MHC Class I antigen signal sequence (ss) and Prolactin signal sequence (Prl), for optogenetic inhibition.

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

ss-Prl-Arch-GFP

Use

Lentiviral

Tags

GFP, Prl, and ss

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
Direct-seq_lentiGuide-Puro-Loop2-8A8G

Plasmid

#157983

Purpose

Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

Depositor

Lijia Ma

Article

Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

Insert

S. pyogenes sgRNA cassette (Loop2-8A8G)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-del-N

Plasmid

#61004

Purpose

Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete N (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 17-310

Promoter

CMV

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pLentiGuide-Puro/CD73-gRNA4

Plasmid

#132588

Purpose

Lentiviral gRNA vector targeting human CD73

Depositor

Robert Sobol

Article

Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

Insert

CD73 (NT5E Human)

Expression

Mammalian

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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INS-GFP-CD19-EPT

Plasmid

#210469

Purpose

Donor plasmid for knock-in GFP-CD19 into the human INS locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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piGECI-NES-N1

Plasmid

#160422

Purpose

Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-del-C

Plasmid

#61005

Purpose

Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete C (PTPN14 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 934-1187

Promoter

CMV

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6P1-Ubl

Plasmid

#78712

Purpose

Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 2-85

Tags

GST-Tag

Expression

Bacterial

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TUBG2

Plasmid

#171966

Purpose

Expression in cell lines of the TUBG2 gene

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG2 (TUBG2 Human)

Expression

Mammalian

Mutation

Removal of the internal TUBG2-EcoRI site by chang…

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-HMGB1

Plasmid

#183195

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

HMGB1

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEB31 (hANGPTL8-V5-His lenti)

Plasmid

#109116

Purpose

V5 and His-tagged human ANGPTL8 in a lentiviral vector

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (ANGPTL8 Human)

Use

Lentiviral

Tags

6x His and V5

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2

Plasmid

#140572

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

Plasmid

#194897

Purpose

Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.

Depositor

Shawn Liu

Article

Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

Inserts

dLbCpf1-NLS-hCTCF_WT
U6-crRNA-scramble

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Human codon optimized catalytically inactive LbCp…

Promoter

TRE and U6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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human ETV6 gRNA-1

Plasmid

#133353

Purpose

human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Expression

Mammalian

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3-Y132D

Plasmid

#113425

Purpose

Expresses hA3A-BE3-Y132D in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Mutation

hAPOBEC3A_Y132D

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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2xmyc-RAB35

Plasmid

#164632

Purpose

Expresses RAB35 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

RAB35 (RAB35 Human)

Tags

2xmyc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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