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pCMV-Tag2B EDD C2768A

Plasmid

#37189

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine

Promoter

CMV

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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pINDUCER22-MKK6E-Flag

Plasmid

#99259

Purpose

Lentiviral vector encoding a doxycycline-inducible Flag-tagged constitutively active MKK6.

Depositor

Alejandro Adam, Peter Vincent

Article

Adam et al PLoS One. 2016 Sep 7;11(9):e0161975. doi

Insert

MKK6E (MAP2K6 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S207E T211E

Promoter

TRE2

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5 EDD C2768A

Plasmid

#37192

Depositor

Darren Saunders, Charles Watts

Article

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine; K503R (please s…

Promoter

SV40

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET28b-SpG-His

Plasmid

#179318

Purpose

Plasmid for bacterial expression and purification of SpG-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpG

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB3-v2

Plasmid

#154894

Purpose

Expresses ERBB3 (a.k.a HER3) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB3 (ERBB3 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

Plasmid

#145840

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-ERBB2-v1

Plasmid

#154901

Purpose

Expresses ERBB2 fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Tags

Venus fragment 1 (v1)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G

Plasmid

#204190

Purpose

Doxycycline inducible lentiviral vector for human TFAP2C overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

TFAP2C (TFAP2C Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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FcD265A-IL2

Plasmid

#65419

Purpose

Mammalian expression plasmid for murine serum-persistent IL-2

Depositor

Dane Wittrup

Article

Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.

Insert

FcD265A-IL2 (Il2 Mouse)

Tags

His

Expression

Mammalian

Mutation

D265A

Promoter

Modified CMV Promoter

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
pDEST-ERBB2-v2

Plasmid

#154895

Purpose

Expresses ERBB2 (a.k.a HER2) fused to Venus fragment 2 (v2) for use in bimolecular fluorescence complementation (BiFC) assays

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

ERBB2 (ERBB2 Human)

Tags

Venus fragment 2 (v2)

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

Plasmid

#42651

Purpose

destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

Depositor

Scott Friedman

Article

Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

Insert

TLR4 399 SNP (TLR4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T399I

Promoter

CMV/TO

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1

Plasmid

#212522

Purpose

Encodes SARS-CoV-2 variant XBB.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGMC00005

Plasmid

#166722

Purpose

sgRNA against human ASNS

Depositor

Raj Chari

Article

Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

Insert

ASNS (ASNS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00006

Plasmid

#166723

Purpose

sgRNA against human ASNS

Depositor

Raj Chari

Article

Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

Insert

ASNS (ASNS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-E55A-ATPIF1

Plasmid

#85404

Purpose

Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase

Depositor

David Sabatini

Article

Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

Insert

ATPIF1 (ATP5IF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E55A point mutation abrogating the ability of ATP…

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
pTwist-SARS-CoV-2 Spike-deltaC18 EG.5.1

Plasmid

#212454

Purpose

Encodes SARS-CoV-2 variant EG.5.1 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike EG.5.1 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53 Δ1-42

Plasmid

#196269

Purpose

Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

p53 Δ1-42 (Trp53 Mouse)

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 1 to 42

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgMettl3

Plasmid

#196263

Purpose

Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 sgRNA (Mettl3 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 XBB

Plasmid

#212521

Purpose

Encodes SARS-CoV-2 variant XBB Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike XBB (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA flag PGC1 (180-797)

Plasmid

#8943

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

PGC-1a (Ppargc1a Mouse)

Tags

flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only