We narrowed to 19,086 results for: iNOS

1. pAAV-Ef1a-Con/Fon-BFP

   Plasmid

   #137129

   Purpose

   Intersectional viral expression of BFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Fon-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS D614 soluble Spike

   Plasmid

   #164652

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

3. GFP-ataxin-3 C14A

   Plasmid

   #89977

   Purpose

   Expresses catalytic inactive GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 14 to Alanine

   Promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST17-ALDH1A3

   Plasmid

   #189750

   Purpose

   The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 (ALDH1A3 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRY2-INPP5E(D556A)

   Plasmid

   #79564

   Purpose

   expression of CRY2PHR fused to catalytically dead INPP5E

   Depositor

   Pietro De Camilli, Olof Idevall-Hagren

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   INPP5E (INPP5E Human)
   

   Use

   Tags

   CRY2 (photolyase homology region domain of Arabid…

   Expression

   Mammalian

   Mutation

   C-terminal region, aa 214_644, C641A mutation to …

   Promoter

   CMV

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pLEX303-ARF6-T157N-mNeonGreen

   Plasmid

   #162028

   Purpose

   Fast cycling ARF mutant

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T151N

   Promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-MAP3K2

   Plasmid

   #85652

   Purpose

   retroviral expression of human MAP3K2

   Depositor

   Julien Sage

   Article

   Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

   Insert

   MAP3K2 (MAP3K2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PGK Puro GFP-GABARAPL1 G116

   Plasmid

   #123243

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted amino acid 117. Stop codon after G116

   Promoter

   PGK

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+-Y276H PHYB-CAAX

   Plasmid

   #154911

   Purpose

   1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

   Depositor

   Jonathan Clarke, Clare Buckley

   Article

   Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

   Insert

   Phytochrome B (PHYB Mustard Weed)
   

   Use

   Xenopus/avian/zebrafish

   Tags

   CAAX membrane moiety and mCherry

   Expression

   Mammalian

   Mutation

   1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

   Promoter

   CMV

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-ITR-PytR(PyOtR)-mCherry

    Plasmid

    #204870

    Purpose

    Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    M. mazei pyrrolysyl tRNA mutant "PyOtR"
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

    Promoter

    U6

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPPI4-Strep-SNAP-CTLA-4-His6

    Plasmid

    #223583

    Purpose

    Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Ef1a-Con/Foff 2.0-mCherry

    Plasmid

    #137133

    Purpose

    Intersectional viral expression of mCherry in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIPZ-bioGEF-SUMO2nc-PURO

    Plasmid

    #208043

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO2nc (SUMO2 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Expression

    Mutation

    Q90P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX304-GFP-v1 IQSEC1 WT

    Plasmid

    #162016

    Purpose

    Expression of IQSEC1 splice variant 1

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX301-RFP-GGA3 NGAT

    Plasmid

    #162031

    Purpose

    Expresses NGAT sequence of GGA3

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    GGA3 NGAT (GGA3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA8-Flag-LARS(1-976aa)

    Plasmid

    #139689

    Purpose

    Expresses N-teminal Flag tagged aa 1-976 of LARS1

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    LARS1 (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX304-GFP-v2 IQSEC1 WT

    Plasmid

    #162017

    Purpose

    Expression of IQSEC1 splice variant 2

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

    Plasmid

    #208960

    Purpose

    A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid

    #208953

    Purpose

    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

    Plasmid

    #208952

    Purpose

    A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge