We narrowed to 8,450 results for: Set

1. Aldh1L1 scFv [N103/31]

   Plasmid

   #190522

   Purpose

   Mammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Aldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. VGlut1 scFv [N28/9]

   Plasmid

   #182084

   Purpose

   Mammalian Expression of VGlut1 scFv. Derived from hybridoma N28/9.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting VGlut1 (Rattus norvegicus) (Slc17a7 Mouse)
   

   Use

   Tags

   HA, His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINSPECT_5'-xrRNA_NLuc_3'-XAP1

   Plasmid

   #197264

   Purpose

   Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)

   Plasmid

   #49060

   Purpose

   Expresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP (chloride-sensitive)

   Expression

   Mammalian

   Mutation

   Cl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…

   Promoter

   CMV

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

   Plasmid

   #197265

   Purpose

   Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Pan-Neurofascin (extracellular) scFv [A12/18]

   Plasmid

   #190485

   Purpose

   Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. VGlut1 scFv [N28/9]

   Plasmid

   #190545

   Purpose

   Mammalian Expression of VGlut1 scFV. Derived from hybridoma N28/9.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   VGlut1 (Rattus norvegicus) recombinant scFV (Slc17a7 Mouse)
   

   Use

   Tags

   HA, 6xHis, Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

   Plasmid

   #113694

   Purpose

   U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Anti-VDAC1 [N152B/23R]

   Plasmid

   #177502

   Purpose

   Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody (VDAC1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

    Plasmid

    #154881

    Purpose

    Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    BCL2 (BCL2 Human)
    miR9/9*-124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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