165,343 results

1. pCS2-Mito-mRFP

   Plasmid

   #169001

   Purpose

   Express Mito (Su9)-mRFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   Mito (Su9)-mRFP (encoding a mitochondrial matrix targeting sequence of subunit 9 of the V0 ATPase Su9 of N. crassa)
   

   Use

   Unspecified

   Tags

   mRFP

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LAMP1-SBP-GFP

   Plasmid

   #120172

   Purpose

   Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   Lysosomal-associated membrane protein 1 (Lamp1 Rat)
   

   Tags

   SBP and eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-Beclin 1

   Plasmid

   #86748

   Purpose

   N-terminal HA-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   human Beclin 1 (BECN1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.DEST.YFP

   Plasmid

   #32683

   Purpose

   Lentiviral cDNA/shRNA gateway destination vectors

   Depositor

   Ming-Sound Tsao

   Article

   Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2R3a-Tq2CFP-OcsT

   Plasmid

   #71268

   Purpose

   Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   Tq2CFP
   

   Use

   Gateway

   Tags

   4xGly linker and octaline synthase terminator

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-PspCas13b-GEMSgRNA2

   Plasmid

   #204768

   Purpose

   pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   guide RNA targeting GEMS m6A sensor mRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. eDrFGFR1

   Plasmid

   #183994

   Purpose

   Optically controlled eDrFGFR1 expression in mammalian cells

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrFGFR1 (FGFR1 Human)
   

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. MMW948: CAG-DmrC-NLS-3xFLAG-VPR

   Plasmid

   #104565

   Purpose

   Mammalian expression vector for DmrC fused to VPR activator

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   DmrC-VPR
   

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSA3_His

   Plasmid

   #202765

   Purpose

   human serum albumin design HSA3, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA3
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid

    #154787

    Purpose

    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

    Depositor

    Robert Singer

    Article

    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

    Insert

    Adenosine deaminse (Adar Fly, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP, V5, and stdMCP

    Expression

    Mammalian

    Mutation

    Synonymized serine to prevent autoediting of ADAR…

    Promoter

    UBC

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Human Myc-XIAP

    Plasmid

    #217627

    Purpose

    Mammalian expression of Myc tagged XIAP

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    XIAP (XIAP Human)
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEntr_Myc-BirA-G3-ER

    Plasmid

    #173814

    Purpose

    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

    Depositor

    Norbert Perrimon

    Article

    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

    Insert

    Myc-BirA-G3-ER
    

    Use

    Gateway shuttle vector

    Tags

    Myc

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPI-IRES-Puro-Ak-ACE2-TMPRSS2

    Plasmid

    #154987

    Purpose

    Lentiviral vector expressing Human ACE2 and TMPRSS2

    Depositor

    Sonja Best

    Insert

    ACE2 and TMPRSS2 (ACE2, TMPRSS2 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-SauriCas9

    Plasmid

    #135964

    Purpose

    Expresses SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB551

    Plasmid

    #74881

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBN41

    Plasmid

    #86716

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-2::GFP
    

    Expression

    Worm

    Available Since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHBS1389 IBB-GFP-mCherry3E

    Plasmid

    #118803

    Purpose

    Fluorescence-based TDP43-dependent splicing reporter

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    Synthetic
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_msfGFP-SEPT7

    Plasmid

    #180315

    Purpose

    mammalian expression of human SEPT7 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN7 (SEPTIN7 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCONR221

    Plasmid

    #216146

    Purpose

    Gateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistance

    Depositor

    Imran Siddiqi

    Article

    Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

    Type

    Empty backbone

    Use

    Cloning backbone

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET15-UbE2D1

    Plasmid

    #15782

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)
    

    Tags

    6x His, T7 tag, and Xpress tag (enterokinase clea…

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-FLEX-GFP

    Plasmid

    #28304

    Depositor

    Edward Boyden

    Insert

    GFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pHTC-HaloTag_WDR91:1-747

    Plasmid

    #210997

    Purpose

    Mammalian expression of full-length human WDR91. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    WDR91:M1-A747 (WDR91 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. pEGFP(N3)-PP1beta

    Plasmid

    #44223

    Depositor

    Angus Lamond, Laura Trinkle-Mulcahy

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP1B (PPP1CB Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. pBABE hBRM

    Plasmid

    #1961

    Depositor

    Robert Kingston

    Article

    Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

    Insert

    BRM (SMARCA2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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28. GFP-RPA194

    Plasmid

    #17660

    Depositor

    Tom Misteli

    Article

    Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

    Insert

    RPA194 (Polr1a Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

29. pAGD236

    Plasmid

    #219497

    Purpose

    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

    Insert

    sfGFP, Eco1 msr, Eco1 RT
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pRham-CALM1

    Plasmid

    #182500

    Purpose

    Bacterial expression of the Homo sapiens calmodulin, CALM1

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    CALM1 (CALM1 Human)
    

    Expression

    Bacterial

    Promoter

    rhamnose

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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