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We narrowed to 20,248 results for: Sele

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Showing: 10891 - 10920 of 20248 results

  2. pcDNA3-FKBP-ICUE3-NLS

    Plasmid
    #138255
    Purpose
    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.
    Depositor
    Jin Zhang
    Publication
    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP-ICUE3-NLS
    Tags
    FKBP, Nuclear localization signal (NLS), cpVenus[…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    138255-plasmid-map-sequence-id-307462

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  3. pcDNA3-mCherry-mCherry-FLARE-Cameleon

    Plasmid
    #123352
    Purpose
    Red-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-mCherry-FLARE-Cameleon
    Tags
    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123352-plasmid-map-sequence-id-307178

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  4. pcDNA3-Venus-Venus-FLARE-Cameleon

    Plasmid
    #123351
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-Venus-FLARE-Cameleon
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123351-plasmid-map-sequence-id-307106

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  5. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid
    #123339
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-cpVenus-FLARE-EKAR-EV
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123339-plasmid-map-sequence-id-307068

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  6. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid
    #123333
    Purpose
    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-mCherry-FLARE-AKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    123333-plasmid-map-sequence-id-306922

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  7. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

    Plasmid
    #140014
    Purpose
    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Publication
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Y126A, Y197F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140014-plasmid-map-sequence-id-297743

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  9. pcDNA3-sACE2v5-sfGFP

    Plasmid
    #145176
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 5
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145176-plasmid-map-sequence-id-292575

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  10. pcDNA3-sACE2v4-sfGFP

    Plasmid
    #145175
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 4
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145175-plasmid-map-sequence-id-292620

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  12. pcDNA3-Q842gp120-8his

    Plasmid
    #100932
    Purpose
    Mammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (Q842.d12) Env
    Tags
    CD5 leader sequence and Purification tag after K5…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100932-plasmid-map-sequence-id-197608

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  13. pcDNA3-JNKsubDD-YPet-NES

    Plasmid
    #84633
    Purpose
    Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    JNKsubDD-YPet-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84633-plasmid-map-sequence-id-176152

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  14. pTE3327

    Plasmid
    #107535
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RVR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K548V, N552R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107535-plasmid-map-sequence-id-207331

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  17. dmBACCS2VL-IRES-dOrai-IRES-GFP

    Plasmid
    #72897
    Purpose
    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP
    Depositor
    Takao Nakata
    Publication
    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dmBACCS2VL-IRES-dOrai-IRES-GFP
    Tags
    IRES-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    72897-plasmid-map-sequence-id-133667

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  18. pBrain-GFP-TACC3KDP(S558A)-shTACC3

    Plasmid
    #59357
    Purpose
    Expresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.
    Depositor
    Stephen Royle
    Publication
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59357-plasmid-map-sequence-id-94887

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  20. pLenti Neo glnA PCC 6803 5x sites native blastR-P2A-GFP

    Plasmid
    #254306
    Purpose
    blastR-P2A-GFP reporter under regulation of CMV minimal promoter and glnA PCC 6803 tiled NtcA binding sites separated by native spacer elements. Requires NtcA chimera for reporter gene expression.
    Depositor
    Samuel McBrayer
    Publication
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    blasticidin_resistance-P2A-GFP
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254306-plasmid-map-sequence-id-505742

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  22. CMV-NanoLuc-BRAF-CAT

    Plasmid
    #194772
    Purpose
    Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.
    Depositor
    Deborah Morrison
    Publication
    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
    Tags
    NanoLuc
    Expression
    Mammalian
    Promoter
    CMV51
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194772-plasmid-map-sequence-id-397073

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  23. CMV-BRAF-REG-Halo

    Plasmid
    #194771
    Purpose
    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.
    Depositor
    Deborah Morrison
    Publication
    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV51
    Available since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194771-plasmid-map-sequence-id-384155

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  24. pcDNA3.1-RIαΔ(D/D+linker)-EGFP

    Plasmid
    #181843
    Purpose
    GFP-tagged PKA RIα subunit lacking the docking/dimerization (D/D) and linker regions; for mammalian expression.
    Depositor
    Jin Zhang
    Publication
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RIα-(D/D+linker)EGFP (PRKAR1A Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of residues 12-112 in PKA RIα.
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181843-plasmid-map-sequence-id-364312

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  25. pcDNA3-AktAR-Kras

    Plasmid
    #123357
    Purpose
    FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.
    Depositor
    Jin Zhang
    Publication
    Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AktAR-Kras
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    123357-plasmid-map-sequence-id-307467

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  26. pEGFP-N1 hMOG-beta2-EGFP

    Plasmid
    #160979
    Purpose
    Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells
    Depositor
    Markus Reindl
    Publication
    Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    160979-plasmid-map-sequence-id-339526

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  27. Epac-S-H159

    Plasmid
    #170347
    Purpose
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_tdBlackcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170347-plasmid-map-sequence-id-336136

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  28. Venus-Bad-4E-pEGFP-C1

    Plasmid
    #166740
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Publication
    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bad-4E (BAD Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166740-plasmid-map-sequence-id-329000

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  29. pcDNA3-Venus-cpVenus-FLARE-ICUE

    Plasmid
    #123355
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-cpVenus-FLARE-ICUE
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123355-plasmid-map-sequence-id-307489

    Enlarge

  30. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

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Showing: 10891 - 10920 of 20248 results