We narrowed to 19,089 results for: iNOS

1. pXPR_BRD003 sgKEAP1-1

   Plasmid

   #74985

   Purpose

   sgKEAP1-1, puromycin selection

   Depositor

   William Hahn

   Article

   Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

   Insert

   KEAP1 (KEAP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

   Plasmid

   #208962

   Purpose

   Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTUU

   Plasmid

   #159749

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

   Plasmid

   #208955

   Purpose

   A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL

   Plasmid

   #119952

   Purpose

   Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

   Inserts

   gpps
   limS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminal truncation of 56 amino acid residues (…

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST17-ALDH1A1

   Plasmid

   #189749

   Purpose

   The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A1 (ALDH1A1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBpuro_KLF4_ER

   Plasmid

   #34609

   Depositor

   Michael Ruppert

   Article

   Foster et al Oncogene. 2005 Feb 24;24(9):1491-500.

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Retroviral

   Tags

   Myc epitope tag and estrogen receptor fusion

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   Jan. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pLEX301-ARF6-T157N-TagRFP

   Plasmid

   #162030

   Purpose

   Fast cycling ARF mutant

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   T151N

   Promoter

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Δ18 K417N+E484K+N501Y

   Plasmid

   #169469

   Purpose

   Encodes SARS-CoV-2 K417N+E484K+N501Y Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 K417N+E484K+N501Y Spike truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. StrKDEL-IRES-mannosidase II-mTagBFP2

    Plasmid

    #165460

    Purpose

    Expressed mannII-BFP simultaneously with a RUSH Hook Str-KDEL.

    Depositor

    David Stephens

    Article

    McCaughey et al J Cell Sci. 2021 Aug 5. pii: 271201. doi

    Insert

    Partial ManII-mTag2BFP fusion (Man2a1 Mouse)
    

    Use

    Tags

    Str-KDEL and mTag2BFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-80L 2xHis-LRRK2 Armadillo 1-159

    Plasmid

    #186016

    Purpose

    2xHis-LRRK2 Armadillo 1-159

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo 1-159 (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    See depositor's comments below.

    Promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE-80L 2xHis-LRRK2 Armadillo 350-550

    Plasmid

    #186018

    Purpose

    2xHis-LRRK2 Armadillo 350-550

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo 350-550 (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-Coff/Fon-sRGECO

    Plasmid

    #137128

    Purpose

    Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Coff/Fon-sRGECO
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E217D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. KCC2-pHext

    Plasmid

    #104076

    Purpose

    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

    Depositor

    Igor Medyna

    Article

    Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Use

    Tags

    pHluorine tag inserted in the second putative tra…

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    UbC

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

    Plasmid

    #137165

    Purpose

    Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    3x-GCaMP6M
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    Removed RSET tag

    Promoter

    Ef1a

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-Con/Foff 2.0-BFP

    Plasmid

    #137130

    Purpose

    Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-BFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114099

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS1062

    Plasmid

    #29011

    Purpose

    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple155-Cre/EGFP-NLS
    

    Use

    High copy number homologous recombination plasmid…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MiniPromoter Ple155 from the human PCP2 gene

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2274

    Plasmid

    #111900

    Purpose

    ssAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple344 (TUBB3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple344 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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