We narrowed to 15,617 results for: nol
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Plasmid#164609PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in astrocytesDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVMutationS383TPromoterGfaABC1DAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB4542
Plasmid#215261PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19DepositorInsertCBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4401
Plasmid#215252PurposeModule for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertSF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZDonor-Nm-Cas9-gRNA-hU6
Plasmid#61366Purposeempty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNADepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhuman U6Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
AA294
Plasmid#215968PurposeFragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoterDepositorHas ServiceCloning Grade DNAInsertU1a_v1
UseFragmentAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA332
Plasmid#216053PurposeFragmid fragment: (C' terminus) 3x NLS sequencesDepositorHas ServiceCloning Grade DNAInsertSV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-ALB_DF_B358c_attB
Plasmid#182146PurposeTo insert attB attachment site at ALB in human cells via twinPEDepositorInsertALB_B358c_attB pegRNA
ExpressionMammalianPromoterhU6Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-ALB_DF_A277c_attB
Plasmid#182145PurposeTo insert attB attachment site at ALB in human cells via twinPEDepositorInsertAlb_A277c_attB pegRNA
ExpressionMammalianPromoterhU6Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS4A
Plasmid#120826PurposeEctopic expression of ZIKV proteinDepositorInsertNS4A ZIKV
TagsV5ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-ChRger2-TS-EYFP
Plasmid#127238PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianPromoterCamKIIaAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TATV)(BB) (pY221)
Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB545
Plasmid#74879PurposeGateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Trx CC32/35SS
Plasmid#36946DepositorInsertThioredoxin 1 (TXN Human)
TagsHis tagExpressionBacterialMutationboth active site cysteines (32,35) mutated to ser…PromoterT7Available SinceMay 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGWB409
Plasmid#74803PurposeGateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdH3proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/NS3
Plasmid#120822PurposeEctopic expression of ZIKV proteinDepositorInsertNS3 ZIKV
TagsV5ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only