We narrowed to 19,449 results for: tec
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Plasmid#216040PurposeFragmid fragment: (C' terminus) for prime editingDepositorHas ServiceCloning Grade DNAInsertMMLV-RT_v1.1; SV40NLS_v1.3
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSP2292
Plasmid#70707PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available SinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)
Plasmid#191164PurposeSimilar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequencesDepositorInsertmCherry:s70587:EGFP (additional cutsites)
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Mito-7
Plasmid#56596PurposeLocalization: Mitochondria, Excitation: 513, Emission: 527DepositorAvailable SinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFP gRNA (BRDN0000562805)
Plasmid#80035Purpose3rd generation lentiviral gRNA plasmid targeting EGFPDepositorInsertEGFP
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
NO- DSD Sensor 2
Plasmid#199786PurposeSynthetic gene circuitDepositorInsertNO- DSD Sensor
UseSynthetic BiologyAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)
Plasmid#140658PurposeOsTIR1(WT)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(WT) mAID-EGFP-Nluc TOL2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cluc-RMA
Plasmid#189624PurposeExpresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.DepositorInsertCypridina luciferase fused to Fc
UseAAV and LuciferaseTagsCluc and IgG1 FcExpressionMammalianPromoterhSynAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTwt
Plasmid#207460PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
vDip-C: pAAV-Pcp2-mGreenLantern-KASH
Plasmid#207613PurposevDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cellsDepositorInsertmGreenLantern
UseAAVTagsKASHPromotermouse Pcp2 promoter (L7-6)Available SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-WT 6KQ
Plasmid#17562DepositorInsertFoxo1 6KQ (Foxo1 Mouse)
TagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailable SinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
TagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
AXBE
Plasmid#204605PurposeMammalian expression of adenine transversion base editor AXBEDepositorInsertbpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationS. pyogenes Cas9 (D10A)Available SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
Plasmid#160611PurposeTranscriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:LbCas12a:T35s
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-MBP-NRBF2
Plasmid#99330PurposeMammalian expression of NRBF2, which associates with the PI3KC3-C1 complexDepositorInsertNuclear receptor-binding factor 2 (NRBF2 Human)
TagsHis-MBPExpressionBacterialPromoterT7Available SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minBG-CI-SaCas9-W3SynpA
Plasmid#231369PurposeminBG-driven SaCas9, with short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.minCMVe-SCP1-SaCas9-W3SynpA
Plasmid#231370PurposeSCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.DepositorInsertSaCas9
UseAAVAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
EK0493 SFFV-mCherry-SGc(GAPDH.s1)cSG-EGFP-(3xMS2) (FLP-IN)
Plasmid#191156PurposeExpression of a sensor for GAPDH 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTRDepositorInsertmCherry:GAPDH.s1:EGFP-3xMS2
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTPH413
Plasmid#185728PurposeBacterial PAM profiling of adenine base editor variantsDepositorInsertsecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA
UseCRISPRExpressionBacterialMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…Available SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
[pSK468] pLJC5 Flag-Depdc5
Plasmid#109337PurposeLenti-viral expression of Flag-tagged wildtype Depdc5DepositorAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only