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pOVC1_X_AsPT1-LOV2-EcSsra

Plasmid

#131217

Purpose

Expresses AsPT1-LOV2-EcSsra (Avena Sativa) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AsPT1-LOV2-EcSsra

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_AsPT1-LOV2-pep

Plasmid

#131215

Purpose

Expresses AsPT1-LOV2-pep (Avena sativa) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AsPT1-LOV2-pep

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD5cmyc2-bglpA

Plasmid

#110645

Purpose

Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CDcmyc2-bglpA

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX-u36_Snifit

Plasmid

#119045

Purpose

Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX-u36_Snifit

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

36TAG R44L H45Q

Promoter

pBAD

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX-u51_Snifit

Plasmid

#119046

Purpose

Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX-u51_Snifit

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

51TAG R44L H45Q

Promoter

pBAD

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-MTX-u23_Snifit

Plasmid

#119044

Purpose

Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)

Depositor

Kai Johnsson

Article

Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-

Insert

MTX-u23_Snifit

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

23TAG R44L H45Q

Promoter

pBAD

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HypD

Plasmid

#110288

Purpose

Expresses t-4-hydroxy-L-proline dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGAD-GH-Mefv iso1

Plasmid

#92107

Purpose

Yeast two hybrid

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 mSesn2 (1190)

Plasmid

#66916

Purpose

bacterial expression of mouse Sesn2

Depositor

Michael Karin, Jun Hee Lee

Article

Park et al Nat Commun. 2014 Jun 20;5:4233. doi: 10.

Insert

Sesn2 (Sesn2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

Plasmid

#91874

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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LLhE_AW-pHG165

Plasmid

#90062

Purpose

Constitutive expression of a chimeric LacI:CelR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhE_AW

Expression

Bacterial

Mutation

I48V/Q55A/Q60R

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhS-pHG165a

Plasmid

#90040

Purpose

Constitutive expression of a chimeric LacI:GalS transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhS

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhF-pHG165a

Plasmid

#90041

Purpose

Constitutive expression of a chimeric LacI:FruR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhF

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a

Plasmid

#90042

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhR-pHG165a

Plasmid

#90043

Purpose

Constitutive expression of a chimeric LacI:RbsR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhR

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a

Plasmid

#90044

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhE-pHG165

Plasmid

#90045

Purpose

Constitutive expression of a chimeric LacI:CelR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhE

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a

Plasmid

#90046

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSI HA Nup53xl

Plasmid

#86000

Purpose

mammalian expression of HA tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

HA

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSI myc Nup53xl

Plasmid

#86001

Purpose

mammalian expression of myc tagged Xenopus laevis Nup53

Depositor

Wolfram Antonin

Article

Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

Insert

Xenopus laevis Nup53 (nup35.L Frog)

Tags

myc

Expression

Mammalian

Mutation

codon optimzed for bacterial expression

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HOMEZ_1

Plasmid

#86352

Purpose

Encodes gRNA for 3' target of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HOMEZ (HOMEZ Human)

Use

CRISPR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF652_2

Plasmid

#86317

Purpose

Encodes gRNA for 3' target of human ZNF652

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF652 (ZNF652 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HOMEZ

Plasmid

#86289

Purpose

Donor vector for 3' FLAG tag of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HOMEZ homology arms (HOMEZ Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HOMEZ_2

Plasmid

#86353

Purpose

Encodes gRNA for 3' target of human HOMEZ

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HOMEZ (HOMEZ Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_TFE3_1

Plasmid

#86325

Purpose

Encodes gRNA for 3' target of human TFE3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TFE3 (TFE3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HBP1_1

Plasmid

#86320

Purpose

Encodes gRNA for 3' target of human HBP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HBP1 (HBP1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF652_1

Plasmid

#86316

Purpose

Encodes gRNA for 3' target of human ZNF652

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF652 (ZNF652 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HBP1

Plasmid

#86272

Purpose

Donor vector for 3' FLAG tag of human HBP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HBP1 homology arms (HBP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pET23a-MmUBB(SnaBI)

Plasmid

#69558

Purpose

In vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (Ubb Mouse)

Expression

Bacterial

Mutation

The two last codons (TATTAA; encoding the last ty…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pONL-N1_ITPKA

Plasmid

#65689

Purpose

Expresses ITPKA-ONL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

ONL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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