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pLH-stsgRNA2.1

Plasmid

#64117

Purpose

Vector for expression of St1 sgRNA2.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA2.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1

Plasmid

#49784

Purpose

Express YOD1 with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

WT

Available Since

Dec. 16, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-LRR

Plasmid

#66937

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV-40

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3_PKA-RIIα

Plasmid

#168492

Purpose

Mammalian expression of mouse PKA regulatory subunit Iiα, untagged.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

mouse PKA regulatory subunit IIα, untagged

Expression

Mammalian

Available Since

May 14, 2021

Availability

Academic Institutions and Nonprofits only

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pZS220

Plasmid

#177642

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K47A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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slc6a3 probe

Plasmid

#53764

Purpose

cRNA probe to detect the slc6a3 transcript

Depositor

Edward Burton

Article

Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

Insert

DAT

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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S5_EcoRV_Ef1a_AscI

Plasmid

#128469

Purpose

Ef1a promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Ef1a promoter

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMB-PMr

Plasmid

#61181

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pJH4733

Plasmid

#178898

Purpose

Pmyo-3 DAF-2 unc-54 3' UTR C.elegans muscle expression of DAF-2

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

DAF-2 (daf-2 Nematode)

Expression

Worm

Promoter

Pmyo-3

Available Since

Jan. 5, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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XE12 Xwnt-11

Plasmid

#16861

Depositor

Randall Moon

Article

Du et al Mol Cell Biol. 1995 May;15(5):2625-34. d

Insert

Xwnt-11 (wnt11b.L Frog)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pBSV2G_PflaB-mEGFPBb

Plasmid

#118234

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mEGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMB-PDESr

Plasmid

#61207

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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2xsspB-mSc-sspB-ITSN1

Plasmid

#114425

Purpose

Translocating ITSN1 to iLID and activate Cdc42

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

ITSN1

Tags

mScarlet

Expression

Mammalian

Mutation

truncated ITSN1 (1159-1509)

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-RNF40i1

Plasmid

#59594

Purpose

Expression of shRNA against human RNF40

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

RNF40 (RNF40 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B56 alpha shRNA 6-3

Plasmid

#10680

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 alpha shRNA (PPP2R5A Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCMB-APr

Plasmid

#61183

Purpose

Fluorescent reporter for apoplast MtBCP1sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-mCherry

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Venus Cam KII alpha mutant T286D

Plasmid

#29429

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Tags

Venus

Expression

Mammalian

Mutation

T286D mutation in CamKIIa sequence

Available Since

July 5, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-YOD1 C160S

Plasmid

#49785

Purpose

Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

C160S

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393

Plasmid

#216382

Purpose

Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Tetraspanin-12 (TSPAN12 Human)

Use

Baculovirus

Tags

6xHis-3C-splitGFP11

Expression

Insect

Promoter

Polyhedrin

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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