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C122-E54: mRuby3

Plasmid

#162899

Purpose

Standard Gateway entry clone encoding mRuby3 fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Ruby3 fluorescent protein

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRB30-GFP

Plasmid

#165481

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and His

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-FLAG-10x His

Expression

Mammalian

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1143

Plasmid

#160869

Purpose

PJ23110-RBS-RsVS-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Vinorine synthase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1142

Plasmid

#160868

Purpose

PJ23110-RBS-FvSAAT-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Alcohol acyltransferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1135

Plasmid

#160866

Purpose

PJ23110-RBS-CAT-taa-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Chloramphenicol Acetyltransferase

Use

Synthetic Biology

Expression

Bacterial

Promoter

PJ23110_BBa_B0029

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLNL1126

Plasmid

#160858

Purpose

PJ23110-RBS-FaAAT2-mRuby2-tL3S2P24_KanR_p15A

Depositor

John Dueber

Article

Latimer et al ACS Synth Biol. 2020 Sep 18. doi: 10.102

Insert

Alcohol acyl transferase

Use

Synthetic Biology

Tags

mRuby2

Expression

Bacterial

Promoter

PJ23110

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-delN-EcGlpG

Plasmid

#158537

Purpose

Bacterial expression of rhomboid protease EcGlpG without N terminus

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

delta N-terminus EcGlpG

Tags

GST tag

Expression

Bacterial

Mutation

N-terminal deletion (1-86)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_GFP_6TS_mCH

Plasmid

#127110

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

GFP-mCherry fusion

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI123

Plasmid

#126063

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x3 (5 aa linker)

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI124

Plasmid

#126064

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

GFP 11x7 (5 aa linker)

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI125

Plasmid

#126065

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

YFP1-10

Use

Synthetic Biology

Tags

linker (GTGGGGS)

Mutation

T205Y

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pClodAcytCh-GFP-P4M

Plasmid

#113346

Purpose

Bacterial expression of Cherry and GFP-SidM-P4M domain (2 copies)

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

Cherry
GFP-SidM-P4M domain (2 copies of P4M in tandem)

Expression

Bacterial

Promoter

ProD and proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pre-Ubc4 BrG pUC19

Plasmid

#111322

Purpose

for transcription to Ubc4 pre-mRNA

Depositor

Christine Guthrie

Article

Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

Insert

UBC4 (UBC4 Budding Yeast)

Expression

Bacterial

Mutation

intron has branch-site A to G mutation

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3MCSpA

Plasmid

#109485

Purpose

Drive inserted gene in osteoblasts.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal DsRedEx2

Plasmid

#104024

Purpose

Plasmid encoding 2ndVal DsRed-Express2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal DsRed-Express2

Use

Low copy

Expression

Bacterial

Mutation

DsRed-Express2 with valine inserted in 2nd positi…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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TM SE T206A

Plasmid

#79664

Purpose

More dimeric SE attached to Triple Mutant GEVI from Piao et al 2015

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Triple Mutant SE T206A

Expression

Mammalian

Mutation

T206A

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJK593

Plasmid

#71587

Purpose

Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag (H6YfdE)

Depositor

T. J. Kappock

Article

Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

Insert

acetyl-CoA:oxalate CoA-transferase (yfdE )

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pKS013

Plasmid

#65466

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

pl-TetO

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
cisRAJ31-cat

Plasmid

#69933

Purpose

Expresses cisRAJ31-cat, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-cat

Use

Synthetic Biology

Expression

Bacterial

Promoter

PlLacO1

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pUG35-YDL183c-GFP

Plasmid

#70161

Purpose

yeast expression of YDL183c with GFP fusion

Depositor

Karin Nowikovsky

Article

Zotova et al J Biol Chem. 2010 May 7;285(19):14399-41

Insert

YDL183c

Tags

yEGFP

Expression

Yeast

Promoter

MET17

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only