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Showing: 7321 - 7340 of 78131 results

  3. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  4. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Publication
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    112139-plasmid-map-sequence-id-217823

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  6. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Catherine Berlot
    Publication
    Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    66080-plasmid-map-sequence-id-436984

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  8. pQCXIP-VEGFA121

    Plasmid
    #73017
    Purpose
    Retroviral expression of human VEGFA121
    Depositor
    Michael Grusch
    Insert
    VEGFA (VEGFA Human)
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    73017-plasmid-map-sequence-id-133914

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  9. pCDH-EF1-DIO-tdTomato

    Plasmid
    #72254
    Purpose
    Express tdTomato under EF-1 promoter when Cre is expressed by another vector
    Depositor
    Kazuhiro Oka
    Insert
    tdTomato
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available since
    Jan. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    72254-plasmid-map-sequence-id-134462

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  17. HAT1

    Plasmid
    #25239
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HAT1_05 (HAT1 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    4 Articles

  20. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    91130-plasmid-map-sequence-id-182254

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Showing: 7321 - 7340 of 78131 results