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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,431 results for: BLI

Showing: 7341 - 7360 of 8431 results

  2. AICSDP-64: FUS-mEGFP

    Plasmid
    #114410
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS
    Depositor
    Allen Institute for Cell Science
    Insert
    FUS Homology Arms with linker-mEGFP (FUS Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114410-plasmid-map-sequence-id-220875

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  4. pDule2-Acd82

    Plasmid
    #174100
    Purpose
    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.
    Insert
    Acridone 82 tRNA synthetase and tRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N311S, C313G, V366A, W382T
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    174100-plasmid-map-sequence-id-345717

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  6. AICSDP-43: GJA1-mEGFP

    Plasmid
    #109120
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1
    Depositor
    Allen Institute for Cell Science
    Insert
    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    May 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    109120-plasmid-map-sequence-id-209766

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  7. pUASp YFP Ras85D CA

    Plasmid
    #52247
    Purpose
    Drosophila expression of YFP-Ras85D constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Ras85D (Ras85D Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pUb-tdTomato-LP-Neo-LP

    Plasmid
    #231509
    Purpose
    Fluorescent reporter for tdTomato expression in Acanthamoeba via the Cre/loxP system
    Depositor
    Yeonchul Hong
    Insert
    tdTomato fluorescent protein
    Use
    Unspecified; Acanthamoeba castellanii neff strain
    Tags
    Expression
    Mutation
    Promoter
    Acanthamoeba polyubiquitin
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231509-plasmid-map-sequence-id-462659

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  11. pGAPDH-Cre-HYG

    Plasmid
    #231510
    Purpose
    Expresses Cre recombinase in Acanthamoeba
    Depositor
    Yeonchul Hong
    Insert
    Cre recombinase
    Use
    Unspecified; Acanthamoeba castellanii neff strain
    Tags
    EGFP and SV40 NLS
    Expression
    Mutation
    Promoter
    Acanthamoeba GAPDH
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231510-plasmid-map-sequence-id-462631

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  12. pET14b-dabAB2

    Plasmid
    #133013
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133013-plasmid-map-sequence-id-262987

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  15. pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

    Plasmid
    #183609
    Purpose
    Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    Neo
    mKate2-3xNLS
    Use
    Mouse Targeting
    Tags
    3xNLS
    Expression
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183609-plasmid-map-sequence-id-361597

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  18. lentiMutB3GALT5catdead-blast

    Plasmid
    #220869
    Purpose
    lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence
    Depositor
    Jennifer Kohler
    Insert
    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    Use
    Lentiviral
    Tags
    myc, FLAG
    Expression
    Mammalian
    Mutation
    encodes D156A and D243A mutations; also, nucleoti…
    Promoter
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220869-plasmid-map-sequence-id-440865

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  19. pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1

    Plasmid
    #122964
    Purpose
    Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 promoter. It can be used as a cloning vector for gene of interest at SfiI sites.
    Depositor
    FENG-QIAN Li, Ken-Ichi Takemaru
    Insert
    human Chibby1 (CBY1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mutation
    Promoter
    FoxJ1 and human FoxJ1
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122964-plasmid-map-sequence-id-291837

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  20. pCCM

    Plasmid
    #162708
    Purpose
    Second CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM cluster
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    Second CCM operon cloned from H. neapolitanus
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162708-plasmid-map-sequence-id-324195

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Showing: 7341 - 7360 of 8431 results