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We narrowed to 7,887 results for: Lif

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Showing: 7341 - 7360 of 7887 results

  10. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  12. pS-sg:GFP

    Plasmid
    #196296
    Purpose
    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding sgRNA:GFP fusion in place of viral NSs
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV S antigenome encoding sgRNA:GFP fusion in place of viral NSs
    Use
    CRISPR
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196296-plasmid-map-sequence-id-389821

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  14. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

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  17. pM-ABE

    Plasmid
    #196292
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding ABE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196292-plasmid-map-sequence-id-390025

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  19. PB-XL-AncBE4max

    Plasmid
    #136254
    Purpose
    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max
    Depositor
    Zhaohui Ye
    Article
    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:
    Insert
    AncBE4max_GFP
    Use
    CRISPR; Piggybac dna transposon
    Expression
    Mammalian
    Mutation
    The AncBE4max_GFP insert was PCR amplified from a…
    Promoter
    TRE3G
    Available Since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136254-plasmid-map-sequence-id-270570

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Showing: 7341 - 7360 of 7887 results