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PX458_KLF11_1

Plasmid

#86314

Purpose

Encodes gRNA for 3' target of human KLF11

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF11 (KLF11 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIER3_1

Plasmid

#86323

Purpose

Encodes gRNA for 3' target of human MIER3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIER3 (MIER3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KDM3A_2

Plasmid

#86322

Purpose

Encodes gRNA for 3' target of human KDM3A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM3A (KDM3A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HMG20A_1

Plasmid

#86318

Purpose

Encodes gRNA for 3' target of human HMG20A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HMG20A (HMG20A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF11_2

Plasmid

#86315

Purpose

Encodes gRNA for 3' target of human KLF11

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF11 (KLF11 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KAT7_1

Plasmid

#86309

Purpose

Encodes gRNA for 3' target of human KAT7

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KAT7 (KAT7 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HMG20A

Plasmid

#86271

Purpose

Donor vector for 3' FLAG tag of human HMG20A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HMG20A homology arms (HMG20A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_KLF11

Plasmid

#86269

Purpose

Donor vector for 3' FLAG tag of human KLF11

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KLF11 homology arms (KLF11 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pJOG250

Plasmid

#80582

Purpose

recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG251

Plasmid

#80583

Purpose

recipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG252

Plasmid

#80584

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pJOG253

Plasmid

#80585

Purpose

recipient plasmid [single activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
pAtU6-BpiI-ccdB_CmR-BpiI_sgRNA_scaffold

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.4

Plasmid

#78539

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.3

Plasmid

#78538

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.3

Plasmid

#78542

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.2

Plasmid

#78537

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL11062

Plasmid

#68256

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-2

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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AP303-4

Plasmid

#66088

Purpose

co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs1

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
AP334-5

Plasmid

#66096

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs5

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
APq5271

Plasmid

#66085

Purpose

co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APa4-2

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only