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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,942 results for: grna

Showing: 7341 - 7360 of 15942 results

  1. pSpec5'

    Plasmid
    #228549
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228549-plasmid-map-sequence-id-454858

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  2. pSpec3'

    Plasmid
    #228548
    Purpose
    Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228548-plasmid-map-sequence-id-454857

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  3. bU6-sgNeo1-mU6-sgLkb1_2nd-hU6-sgNT

    Plasmid
    #177229
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgLkb1_2nd/sgNT1
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177229-plasmid-map-sequence-id-351963

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  4. bU6-sgLkb1_1st-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177225
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNeo1/sgNT1
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177225-plasmid-map-sequence-id-351956

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  5. bU6-sgNeo1-mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177226
    Purpose
    Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177226-plasmid-map-sequence-id-351955

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  6. bU6-sgLkb1_2nd-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177228
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_2nd/sgNeo1/sgNT1
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177228-plasmid-map-sequence-id-351921

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  7. pCASCADE-udhA-zwf

    Plasmid
    #87153
    Purpose
    udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87153-plasmid-map-sequence-id-174933

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  11. GG-EBNA-MIR302-3g-EEA-2g-PGK-Puro

    Plasmid
    #201677
    Purpose
    EBNA episome plasmid for U6 promoter-driven expression of 3 gRNAs targeting miRNA302/367 (Addgene #201960) and 2 gRNAs targeting EEA-motif (Addgene #102898). Includes PGK-puro selection cassette
    Depositor
    Timo Otonkoski
    Article
    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-
    Insert
    MIR302-3g-EEA-2g-PGK-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    June 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201677-plasmid-map-sequence-id-395330

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  14. pAGK62

    Plasmid
    #228122
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available Since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228122-plasmid-map-sequence-id-455403

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  16. pAGK111

    Plasmid
    #228126
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228126-plasmid-map-sequence-id-455418

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Showing: 7341 - 7360 of 15942 results