We narrowed to 4,504 results for: pcr

1. L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1

   Plasmid

   #219697

   Purpose

   Level 0 to construct history-dependent target

   Depositor

   Jennifer Nemhauser

   Insert

   GG0-tUBQ1-NLS-NeonGreen-GG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6

   Plasmid

   #219693

   Purpose

   Level 0 to construct history-dependent target T2PB

   Depositor

   Jennifer Nemhauser

   Insert

   GG4-Bxb1B-PhiC31B-GG6
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. L0I80_Bxb1mut(noBsaI)_GG1-GG4

   Plasmid

   #219715

   Purpose

   Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.

   Depositor

   Jennifer Nemhauser

   Insert

   GG1-Bxb1(noBsaI0)-GG4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVITRO1-dV-IgG1/κ

   Plasmid

   #52213

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin kappa constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVITRO1-dV-IgG1/λ

   Plasmid

   #52214

   Purpose

   Human immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   Human immunoglobulin gamma 1 constant region
   Human immunoglobulin lambda constant region
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deleted Variable region

   Promoter

   Mouse Elongation Factor 1 Alpha and Rat Elongatio…

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Seattle_IPDA_control_1_001

   Plasmid

   #167347

   Purpose

   ddPCR gating control for droplets containing all 5 targets (2 in pol)

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   pol_gag_tat_env
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Seattle_IPDA_control_9_003

   Plasmid

   #167349

   Purpose

   ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   gag_tat_env
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Seattle_IPDA_control_6_002

   Plasmid

   #167348

   Purpose

   ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   pol_gag_tat
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Seattle_IPDA_control_29_006

   Plasmid

   #167352

   Purpose

   ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   pol_env
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Seattle_IPDA_control_14_004

    Plasmid

    #167350

    Purpose

    ddPCR gating control for droplets containing the env target

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Seattle_IPDA_control_32_007

    Plasmid

    #167353

    Purpose

    ddPCR gating control for droplets containing 5'pol or 3'pol targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Seattle_IPDA_control_17_005

    Plasmid

    #167351

    Purpose

    ddPCR gating control for droplets containing gat and tat targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    gat_tat
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR P2R-P3-IRES_ECFP

    Plasmid

    #48354

    Purpose

    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    IRES_ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    ECFP translation is mediated by an IRES. Contains…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid

    #219712

    Purpose

    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.

    Depositor

    Jennifer Nemhauser

    Insert

    GG4-pGATA23-Bxb1-tUBQ10-GG6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L0-T7 Circuit 2 GG1-PhiC31P-bbs1-BxB1P-GG2

    Plasmid

    #219692

    Purpose

    Level 0 to construct history-dependent target T2BP

    Depositor

    Jennifer Nemhauser

    Insert

    GG1-PhiC31P-bbs1-BxB1P-GG2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. L0I89-pMUTE_GG0-1

    Plasmid

    #219708

    Purpose

    Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pMUTE-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. L0I74-_tUBQ10_GG4-GG6

    Plasmid

    #219713

    Purpose

    Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG4-tUBQ10-GG6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT terminator with added golden gate linkers

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid

    #219709

    Purpose

    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pSPCH:PhiC31:tUBQ1-GG4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L0I87-SPCHPromoter_GG0-1

    Plasmid

    #219707

    Purpose

    Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pSPCH-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. L0I84 - GG0-pAHP6-GG1

    Plasmid

    #219706

    Purpose

    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pAHP6-GG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    WT promoter with added golden gate linkers

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2

    Plasmid

    #219691

    Purpose

    Level 0 to construct history-dependent target T2PB

    Depositor

    Jennifer Nemhauser

    Insert

    GG1-Bxb1P-bbs1-PhiC31P-GG2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pScarlessHD-sfGFP-DsRed

    Plasmid

    #80811

    Purpose

    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    sfGFP-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pScarlessHD-3xFLAG-DsRed

    Plasmid

    #80820

    Purpose

    Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    3xFLAG-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pScarlessHD-2xHA-DsRed

    Plasmid

    #80822

    Purpose

    Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    2xHA-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    2xHA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

    Plasmid

    #219711

    Purpose

    Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

    Depositor

    Jennifer Nemhauser

    Insert

    GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJK614

    Plasmid

    #72387

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylamine-glycine ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    contains 4 silent mutations in codons 9-11 and 42…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK621

    Plasmid

    #72388

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylamine-glycine ligase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    contains 3 silent mutations in codons 9-11 to fac…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK592

    Plasmid

    #72386

    Purpose

    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylamine-glycine ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains 3 silent mutations in codons 9-11 to fac…

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLXIN2-EGFP

    Plasmid

    #99204

    Purpose

    EGFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    EGFP
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    A Kozak sequence was inserted 5' to ATG to …

    Promoter

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge