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Showing: 1801 - 1850 of 8301 results

  3. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid
    #153446
    Purpose
    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.CohE
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153446-plasmid-map-sequence-id-306487

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  5. pTXB1-TP1

    Plasmid
    #160084
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein, no linker
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160084-plasmid-map-sequence-id-314364

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  6. pTXB1-TP2

    Plasmid
    #160085
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160085-plasmid-map-sequence-id-313737

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  9. TAK4

    Plasmid
    #227094
    Purpose
    Luciferase reporter vector with MMTV 5' Element cloned downstream of luciferase gene in pHMRΔeluc
    Depositor
    Farah Mustafa
    Article
    Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:
    Insert
    MMTV insert spanning from R region to 400 bp of Gag
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    227094-plasmid-map-sequence-id-456651

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  10. pCS2-MfnG-EcYRS-EGFP*

    Plasmid
    #191119
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Use
    Tags
    His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191119-plasmid-map-sequence-id-373722

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  11. Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2

    Plasmid
    #218206
    Purpose
    Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector
    Depositor
    Katarzyna Koltowska
    Article
    Panara et al Development. 2024 May 1;151(9):dev202525
    Insert
    +15.2prox1a
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218206-plasmid-map-sequence-id-442477

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  16. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  18. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

    Plasmid
    #218205
    Purpose
    Zebrafish -2.1prox1a enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector
    Depositor
    Katarzyna Koltowska
    Article
    Panara et al Development. 2024 May 1;151(9):dev202525
    Insert
    -2.1prox1a
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    218205-plasmid-map-sequence-id-442144

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  27. pGFP AH2 tensin

    Plasmid
    #50514
    Purpose
    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Article
    Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.
    Insert
    Tensin (TNS1 Chicken)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    AA659--761, including actin homology 2 domain of …
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

    Plasmid
    #218207
    Purpose
    Zebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector
    Depositor
    Katarzyna Koltowska
    Article
    Panara et al Development. 2024 May 1;151(9):dev202525
    Insert
    -2.1prox1a_1
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218207-plasmid-map-sequence-id-442476

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  30. Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2

    Plasmid
    #218208
    Purpose
    Zebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector
    Depositor
    Katarzyna Koltowska
    Article
    Panara et al Development. 2024 May 1;151(9):dev202525
    Insert
    -2.1prox1a_2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218208-plasmid-map-sequence-id-442468

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  32. pLV-HKGT4_short_mCherry2-NLS-PEST_Puro

    Plasmid
    #213774
    Purpose
    Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.
    Depositor
    Gaetano Gargiulo
    Article
    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1
    Insert
    HKGT4_short_mCherry2-NLS-PEST
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213774-plasmid-map-sequence-id-421771

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  34. pLV-HKGT3_short_mCherry2-NLS-PEST_Puro

    Plasmid
    #213772
    Purpose
    Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.
    Depositor
    Gaetano Gargiulo
    Article
    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1
    Insert
    HKGT3_short_mCherry2-NLS-PEST
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213772-plasmid-map-sequence-id-421752

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  36. pLV-HKGT2_short_mCherry2-NLS-PEST_Puro

    Plasmid
    #213770
    Purpose
    Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.
    Depositor
    Gaetano Gargiulo
    Article
    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1
    Insert
    HKGT2_short_mCherry2-NLS-PEST
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213770-plasmid-map-sequence-id-421745

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  38. pLV-HKGT1_short_mCherry2-NLS-PEST_Puro

    Plasmid
    #213768
    Purpose
    Fluorescent reporter designed on shortlist of Housekeeping-like signature and transcription factor genes from Tabula Sapiens and Tabula Muris databases.
    Depositor
    Gaetano Gargiulo
    Article
    Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1
    Insert
    HKGT1_short_mCherry2-NLS-PEST
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213768-plasmid-map-sequence-id-421742

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  41. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid
    #153439
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153439-plasmid-map-sequence-id-306439

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  42. pAS_4xMma PylT_FLAG-PcKRS

    Plasmid
    #140022
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    PcKRS (pylS Methanosarcina bakeri)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    M241F, A267S, Y271C, L274M
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140022-plasmid-map-sequence-id-297729

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  43. pAS_4xEcoLeuT(CUA)_AnapRS

    Plasmid
    #140019
    Purpose
    Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    EcoLeuRS (AnapRS)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…
    Promoter
    EF1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140019-plasmid-map-sequence-id-297114

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  47. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  48. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Expression
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

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  49. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid
    #140021
    Purpose
    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006
    Insert
    AcKRS-3 (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    L301M, L305I, Y306F, L309A, C348F
    Promoter
    EF1
    Available since
    May 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    140021-plasmid-map-sequence-id-297663

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  50. pX020

    Plasmid
    #167146
    Purpose
    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal caffeoylpiruvate hydrolase, nnCPH
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167146-plasmid-map-sequence-id-335229

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Showing: 1801 - 1850 of 8301 results