Addgene

We narrowed to 13,026 results for: Mpl

Showing: 721 - 740 of 13026 results

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pBIG1e

Plasmid

#80615

Purpose

pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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eGFP L138 gRNA

Plasmid

#119133

Purpose

gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L138 gRNA

Use

CRISPR

Tags

Expression

Mutation

None

Promoter

U6

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV2-hNCOA6:V5

Plasmid

#63585

Purpose

A subunit of the Trithorax-related (Trr) methyltransferase complex.

Depositor

Kenneth Irvine

Article

Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

Insert

NCOA6 (NCOA6 Human)

Use

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
pPpT4- TEF2prom-TIR1F74G-FLAG

Plasmid

#189727

Purpose

Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF2

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_hNANOG

Plasmid

#112639

Purpose

Plasmid for PCR amplification of hNANOG DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

hNANOG (NANOG Human)

Use

Pcr template

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSpyTag-GlpG

Plasmid

#79054

Purpose

Expresses SpyTagged GlpG protein

Depositor

James Bowie

Article

Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

Insert

SpyTag-GlpG (glpG )

Use

Tags

6xHis and SpyTag

Expression

Bacterial

Mutation

GlpG protein employed here corresponds to residue…

Promoter

Trc

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub5

Plasmid

#201738

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) epsilon-tubulin Tub5 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub5

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub3-mScarlet

Plasmid

#201735

Purpose

Tardigrade (Hypsibius exemplaris) gamma-tubulin Tub3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin gamma

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D4-mScarlet

Plasmid

#201734

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D4 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D4

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D3-mScarlet

Plasmid

#201733

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D3

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D2-mScarlet

Plasmid

#201732

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D2 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D2

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2C-mScarlet

Plasmid

#201731

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2C tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta C

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1C1

Plasmid

#201730

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1C1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1C1

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1A2

Plasmid

#201729

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A2 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A2

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmEGFP-tHe-Tub1A1

Plasmid

#201728

Purpose

mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A1 for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

tHe-Tub1A1

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

HTA1

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

TEF2

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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L1_NOP1:mS-N7-Ck2

Plasmid

#136126

Purpose

L1 in position 2, example of weak promoter

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpNOP1:mScarletI-N7_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19 gene 60 thru gap + Cys

Plasmid

#49161

Purpose

Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Use

Tags

Expression

Mutation

gene 60 mRNA truncated two codons after coding ga…

Promoter

T7

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only

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