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T1ACR1_EYFP_pcDNA3.1

Plasmid

#161027

Purpose

Expresses strongly red-shifted T1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

T1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Nck1 (SH2 mut)

Plasmid

#45893

Depositor

Louise Larose

Article

Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

Insert

Nck-1 SH2 mutant (NCK1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

in SH2 domain (R308K) and E302K and Q333H

Promoter

CMV

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pUC57 iLOV ANAB

Plasmid

#26770

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Cloning vector

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only
pQE30 NFAT5 DBD

Plasmid

#13629

Depositor

Anjana Rao

Article

Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

Insert

NFAT5 (NFAT5 Human)

Tags

6x His

Expression

Bacterial

Mutation

DNA binding domain: aa 175 to 471

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-H2A2B

Plasmid

#106419

Purpose

H-RAN-H2A2B (RANbody H2A2B::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-H2A2B

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCDW115

Plasmid

#70062

Purpose

artificial transcription template "pause ablation"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' A+2G T+6G - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-AO7/2537

Plasmid

#11470

Depositor

Allan Weissman

Article

Lorick et al Proc Natl Acad Sci U S A. 1999 Sep 28. 9

Insert

AO7 (Rnf25 Mouse)

Tags

HA

Expression

Mammalian

Mutation

n/a

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
PL06008A2C06 (smedwi2)

Plasmid

#35698

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi2

Promoter

T7 sense, T3 antisense

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-M-RAN-RFP6

Plasmid

#106428

Purpose

M-RAN-RFP6 (RANbody RFP6::Sm-MYCtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

M-RAN-RFP6

Tags

HA, His, and Multiple MYC

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRAJ31f

Plasmid

#39257

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no terminator for the transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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UbcH10 DN

Plasmid

#8505

Depositor

Joan Ruderman

Article

Townsley et al Proc Natl Acad Sci U S A 1997 Mar 18;94(

Insert

UbcH10 (UBE2C Human)

Expression

Bacterial

Mutation

C114S, L118S

Available Since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
PgACR1_EYFP_pcDNA3.1

Plasmid

#161028

Purpose

Expresses PgACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

PgACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGBT-PX

Plasmid

#31824

Depositor

Stephen Ekker

Article

Petzold et al Proc Natl Acad Sci U S A. 2009 Nov 3;106

Type

Empty backbone

Use

Cre/Lox; Gene trap

Tags

GFP GM2 variant

Promoter

beta Actin

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pGSTag DLC2

Plasmid

#24270

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC2 (Dynll2 Mouse)

Tags

Gst

Expression

Bacterial

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pGL3con-miR15aBul

Plasmid

#21117

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pHL662

Plasmid

#37636

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

mCherry
GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pTH07

Plasmid

#70063

Purpose

artificial transcription template "distal pause"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only
piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only