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pLX307_CdTag-HA_EmptyVector

Plasmid

#221467

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLX302_CdTag-HA_EmptyVector

Plasmid

#221465

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_GFP_CdTag-HA

Plasmid

#221492

Purpose

GFP lentiviral overexpression of C-terminal dTag fusion

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pC4EN-F1-GAFm

Plasmid

#39870

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Insert

iRFP protein GAF domain (mutated)

Use

Tags

FKBP

Expression

Mammalian

Mutation

F165Y, D232Y, W308R

Promoter

CMV

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
YIplac204-pOst1-ESCargo(Crimson)

Plasmid

#140154

Purpose

Expresses a vacuolar cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

pOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI1

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Mutation

Promoter

CBh

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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CF-Ins54p

Plasmid

#20155

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-Inp54p (INP54 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Stop codon at position 331.

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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PX854

Plasmid

#62886

Purpose

C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa573-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Mutation

Promoter

CBh

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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YF-Tiam1

Plasmid

#20154

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Tiam1 (TIAM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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CF-iSH

Plasmid

#20159

Depositor

Tobias Meyer

Article

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Insert

CFP-FKBP-p85 (Pik3r2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pINT-PuroDDEYFP

Plasmid

#31442

Depositor

Gerhart Ryffel

Article

Waldner et al BMC Res Notes. 2011 Oct 18;4:420.

Insert

enhanced yellow fluorescent protein

Use

Phic31/attb

Tags

FKBP L106P

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pERB109

Plasmid

#58280

Purpose

constitutive miRFKBP5_Mis12-GFP-FKBP3; inducible mCh-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
Mis12-GFP-FKBPx3
inducible mCh-FRB

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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pERB130

Plasmid

#58284

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pERB127

Plasmid

#58283

Purpose

const. Mis12-GFP-FKBP3; ind. FRB-mCH(FseI)

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-GFP-FKBPx3
inducible FRB-mCh
FKBP-targeted miRNA

Use

Tags

GFP and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pERB160

Plasmid

#58285

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1
FKBP-targeted miRNA

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pERB122

Plasmid

#58282

Purpose

const: miRFKBP5_GFP_IRES_Puro; ind: mC-FRB

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

FKBP-targeted miRNA
GFP
Puromycin resistance
inducible mCherry-FRB

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only

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iXFGFR4k

Plasmid

#31260

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR4-ICD-FKBP (fgfr4-a Frog)

Use

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pERB171

Plasmid

#58289

Purpose

const. Mis12-FKBP3; ind. FRB-mCH-Mad1^485-718

Depositor

Michael Lampson

Article

Ballister et al J Cell Biol. 2014 Mar 17;204(6):901-8. d

Inserts

Mis12-FKBPx3
inducible FRB-mCh-Mad1^485-718
FKBP-targeted miRNA

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge