We narrowed to 5,482 results for: pcas

1. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

   Plasmid

   #172224

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-p21_S146A

   Plasmid

   #40205

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S146A

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAT.011

   Plasmid

   #119555

   Purpose

   Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-F-API5

   Plasmid

   #157662

   Purpose

   Expresses API5 in mammalian cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Apoptosis inhibitor 5 (API5 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-Bmp15

   Plasmid

   #163594

   Purpose

   Expresses BMP-15 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Bmp15 (Bmp15 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAR-OF reporter

   Plasmid

   #16627

   Depositor

   Bert Vogelstein

   Article

   Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

   Insert

   CAR-OF
   

   Use

   Tags

   beta-galactosidase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-p21_S146D

   Plasmid

   #40206

   Depositor

   Philipp Mergenthaler

   Article

   Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S146D

   Promoter

   CAG

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSW177 - pCASP1

   Plasmid

   #115986

   Purpose

   entry vector for GreenGate cloning method, promoter

   Depositor

   Alexis Maizel

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pCASP1
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGGs-Hoxd10

   Plasmid

   #45721

   Depositor

   Jeremy Dasen

   Article

   Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

   Insert

   Hoxd10 (HOXD10 Chicken)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CMV / b-actin

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCAG/hArf4(WT)-HA

    Plasmid

    #79403

    Purpose

    Expresses C-terminally HA-tagged Arf4(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

    Insert

    Arf4 (ARF4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

11. SpCas9 dFE

    Plasmid

    #190137

    Purpose

    Expresses SpCas9-T4pol (dFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9-T4pol (dFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-Gdf7

    Plasmid

    #163597

    Purpose

    Expresses GDF-7 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf7 (Gdf7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Some Guanin and Cytosine were substituted to redu…

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCA-T-intron-G

    Plasmid

    #36887

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. peSpCas9-J23109

    Plasmid

    #113152

    Purpose

    Plasmid constitutively expressing eSpCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K810A & K1003A & R1060A

    Promoter

    Available since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCA-G-intron-T

    Plasmid

    #36885

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGs-Hoxc8

    Plasmid

    #45555

    Depositor

    Jeremy Dasen

    Article

    Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

    Insert

    Hoxc8 (HOXC8 Chicken)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-GW-V5-Hygro

    Plasmid

    #68265

    Purpose

    Constitutive selectable expression of C-term V5 tagged proteins

    Depositor

    Mitchell Guttman

    Article

    McHugh et al Nature. 2015 May 14;521(7551):232-6. doi

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-DN-RFX

    Plasmid

    #52296

    Purpose

    Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Dominant negative RFX
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS_2paCRY2(D387A)

    Plasmid

    #129652

    Purpose

    2P-activatable CRY2 with the FAD-disrupting mutation

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2(D387A)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCaST-dVP16AD

    Plasmid

    #15306

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    dVP16AD-ZIP+
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge