We narrowed to 5,798 results for: pcas

1. KA2601_eSpCas9-G2P

   Plasmid

   #124203

   Purpose

   Expression of eSpCas9(1.1) and sgRNA

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AV44_pCAG.Cas9-D10A.gRNA.S1

   Plasmid

   #199258

   Purpose

   Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9-D10A nickase
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-HA-dSpCas9

   Plasmid

   #92112

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9
   

   Use

   CRISPR

   Tags

   HA tag and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAFNF-tdTomato

   Plasmid

   #125575

   Purpose

   Flp-dependent expression of tdTomato in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   tdTomato
   

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-Gdf9

   Plasmid

   #163599

   Purpose

   Expresses GDF-9 in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Gdf9 (Gdf9 Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-Rad18

   Plasmid

   #119003

   Purpose

   Mammalian expression of human Rad18 and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Rad18
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCaSpeR-act5cB-QF#7

   Plasmid

   #46125

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7
   

   Expression

   Insect

   Available Since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-T3-st1CAS9-pA

   Plasmid

   #131542

   Purpose

   Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

   Insert

   Codon optimized st1Cas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAR-IF reporter

   Plasmid

   #16628

   Depositor

   Bert Vogelstein

   Article

   Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

   Insert

   CAR-IF
   

   Tags

   beta-galactosidase

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-V5-NLGN2

    Plasmid

    #82538

    Purpose

    pCAG vector for expressing V5-NLGN2

    Depositor

    Alice Ting

    Article

    Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

    Insert

    NLGN2 (Nlgn2 Rat)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-mWasabi-ER

    Plasmid

    #164165

    Purpose

    Expresses mWasabi fused with an ER-targeting sequence of calreticulin and the ER retrieval sequence, KDEL, under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mWasabi
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SpCas9 TadCBEb

    Plasmid

    #193832

    Purpose

    Express TadCBEb (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDb-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCas9CR4-VQR

    Plasmid

    #120224

    Purpose

    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

    Depositor

    Christopher Reisch

    Insert

    SpCas9-VQR
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Mutation

    Mutations D1135V/R1335V/T1337R

    Promoter

    pTet

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCasper4-QF#7-hsp70

    Plasmid

    #46135

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-mTurbo-G

    Plasmid

    #187176

    Purpose

    Express miniTurbo under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

    Insert

    miniTurbo (BirA mutant)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAndy-NBioTAP

    Plasmid

    #53571

    Purpose

    Plasmid vector carrying N-Bio-1xproteinA tag

    Depositor

    Mitzi Kuroda

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAndy-CBioTAP

    Plasmid

    #53570

    Purpose

    Plasmid vector carrying C-Bio-1xproteinA tag

    Depositor

    Mitzi Kuroda

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-p21_S146A

    Plasmid

    #40205

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Expression

    Mammalian

    Mutation

    S146A

    Promoter

    CAG

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-synPCB2.0

    Plasmid

    #139477

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Expression

    Mammalian

    Mutation

    V413L

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAR-OF reporter

    Plasmid

    #16627

    Depositor

    Bert Vogelstein

    Article

    Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

    Insert

    CAR-OF
    

    Tags

    beta-galactosidase

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only