We narrowed to 4,840 results for: phy

1. pGEM-T Easy-AtpU6-26

   Plasmid

   #160218

   Purpose

   AtpU6-26 Golden Gate level 0 piece to express gRNAs

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   pAtU6-26 promoter
   

   Use

   Golden gate level 0 piece to express grnas

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HPV-31neo

   Plasmid

   #153283

   Purpose

   Neomycin selectable HPV31 genome

   Depositor

   Elliot Androphy

   Article

   Gilson et al J Virol. 2020 Jul 30;94(16). pii: JVI.00

   Insert

   HPV-31 genome with Neo cassette
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   HindIII and CsiI site mutated from original Neo c…

   Promoter

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FLAG-Fos DD

   Plasmid

   #8968

   Depositor

   John Blenis

   Article

   Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

   Insert

   c-Fos (Fos Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S362D, S374D

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. Geph CR shRNA

   Plasmid

   #121068

   Purpose

   shRNA targeting the coding region of the gephyrin mRNA

   Depositor

   Angel de Blas

   Article

   Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

   Insert

   GPHN shRNA (Gphn Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC1.530

   Plasmid

   #205441

   Purpose

   Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Insert

   RepB gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Geph CR3m shRNA

   Plasmid

   #121069

   Purpose

   Negative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNA

   Depositor

   Angel de Blas

   Article

   Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

   Insert

   GPHN shRNA (Gphn Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBR5pemIK-GW

   Plasmid

   #126556

   Purpose

   Broad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selection, Gateway cloning compatible

   Depositor

   Lindsey Burbank, Drake Stenger

   Article

   Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pASKt15C+SrtA5

   Plasmid

   #64985

   Purpose

   Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

   Insert

   sortase A
   

   Use

   Tags

   His6 tag and S tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, P94R, D160N, D165A, K19…

   Promoter

   tetracycline promoter/operator

   Available since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. 141_pT3TS_iCre

    Plasmid

    #171793

    Purpose

    Plasmid for optimised iCre RNA synthesis using T3 promoter.

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised Cre for RNA expression
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZA31

    Plasmid

    #158509

    Purpose

    In planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1-4xMyc
    

    Use

    Tags

    4xMyc

    Expression

    Plant

    Mutation

    L17E

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTd-4-15-13-10

    Plasmid

    #63835

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pASKt15C+SrtA7woH

    Plasmid

    #65020

    Purpose

    Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, E105K, E108A, D16…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-Fos AA

    Plasmid

    #8967

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362A, S374A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pmirGLO-Smad5-1719

    Plasmid

    #49382

    Purpose

    Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

    Depositor

    Martine Roussel

    Article

    Murphy et al Cancer Res. 2013 Oct 21.

    Insert

    Smad5 (Smad5 Mouse, Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Human phosphoglycerate kinase (PGK) promoter

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Geph UTR shRNA

    Plasmid

    #121070

    Purpose

    shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pZA13

    Plasmid

    #158491

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    no STOP codon

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG-Fos Y345A

    Plasmid

    #8970

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y345A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-FLAG-Fos F343A

    Plasmid

    #8969

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F343A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only