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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,163 results for: tef

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Showing: 721 - 740 of 3163 results

  1. pAAH2

    Plasmid
    #41826
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41826-plasmid-map-sequence-id-69077

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  3. pRKO

    Plasmid
    #49456
    Purpose
    loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions
    Depositor
    Marc Gartenberg
    Publication
    Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1
    Insert
    loxP-kanMX-loxP cassette
    Use
    Cre/Lox
    Expression
    Bacterial
    Promoter
    none
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49456-plasmid-map-sequence-id-73667

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  14. TRP1MX6-ins4

    Plasmid
    #195041
    Purpose
    pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Publication
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    TRP1
    Use
    Yeast genomic targeting
    Tags
    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195041-plasmid-map-sequence-id-384244

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  15. pCdOpt-BMX

    Plasmid
    #203929
    Purpose
    This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae
    Depositor
    Scott Briggs
    Publication
    Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11
    Insert
    BleMX
    Expression
    Yeast
    Mutation
    BleMX was codon optimized for Candida albicans or…
    Available since
    Dec. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    203929-plasmid-map-sequence-id-414816

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  17. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Publication
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63791-plasmid-map-sequence-id-106478

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  19. pFA6a-CLIP-KanMX6

    Plasmid
    #87025
    Purpose
    Tag S. pombe genes in C terminal with CLIP
    Depositor
    Julien Berro
    Insert
    CLIP
    Expression
    Bacterial and Yeast
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87025-plasmid-map-sequence-id-172996

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  20. THI VP64 pALD4 T-

    Plasmid
    #161481
    Purpose
    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Publication
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161481-plasmid-map-sequence-id-324351

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Showing: 721 - 740 of 3163 results