We narrowed to 9,444 results for: pho

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pMXs-puro HA-Atg14*dCC

Plasmid

#38266

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Promoter

LTR

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
GFP-CLCb (EED/QQN)

Plasmid

#47422

Depositor

Peter McPherson

Article

Poupon et al Proc Natl Acad Sci U S A. 2008 Jan 8;105

Insert

CLCb EED/QQN (CLTB Human)

Tags

EGFP

Expression

Mammalian

Mutation

EED changed to QQN, in aa20-41 region

Promoter

CMV

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA HA-Gnat1 WT-3UTR

Plasmid

#184026

Purpose

Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe 3xFLAG human Bmf

Plasmid

#17242

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (BMF Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
LENTICRISPR-HK2_sgRNA2

Plasmid

#201597

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

Plasmid

#124278

Purpose

Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Mutation

R201A

Promoter

AOX1

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

Plasmid

#107730

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

Plasmid

#107729

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP2A-FBAR-mRFP

Plasmid

#168502

Purpose

Expression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.

Depositor

Franck Polleux

Article

Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

Insert

SRGAP2A-FBAR (SRGAP2 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-dKARD

Plasmid

#114053

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-dKARD (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance): AGATACTAGCT…

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe 3xFLAG mouse Bmf

Plasmid

#17243

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf (Bmf Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP Kind2 S181E

Plasmid

#105312

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181A

Plasmid

#105307

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGK-hMIGA2-DsRed

Plasmid

#192867

Purpose

Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Codon-optimized human Mitoguardin 2 (MIGA2 Human)

Tags

DsRed

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pFLAG R106L-CPTP

Plasmid

#170744

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

FLAG

Expression

Mammalian

Mutation

R106L

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG K60A-CPTP

Plasmid

#170743

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Nature. 2013 Aug 22;500(7463):463-7. doi

Insert

CPTP (CPTP Human)

Tags

FLAG

Expression

Mammalian

Mutation

K60A

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Ly6G-miR30

Plasmid

#163358

Purpose

Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker.

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

Ly-6G (Ly6g Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

Plasmid

#107731

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMY-Ly6G-Rluc_miR

Plasmid

#163339

Purpose

Retroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

Ly-6G (Ly6g Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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