We narrowed to 7,581 results for: ef1a
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Plasmid#118018PurposeConstitutive expression of Cas9DepositorInsertCas9
UseLentiviralExpressionMammalianPromoterEF1alphaAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS
Plasmid#162770PurposeProtein expression in CHO cellsDepositorTypeEmpty backboneExpressionMammalianPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAX198
Plasmid#173042PurposeCustom vector for sgRNA library constructionDepositorInsertHBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
UseCRISPR and LentiviralExpressionMammalianPromotermouse U6Available SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgRNA-EF1α-sakkhCas9-HA-NLS-polyA-CBh-EGFP-polyA
Plasmid#168305Purposemediated knock-in of sgRNA precursorDepositorInsertSaKKH-EGFP-U6-sgRNA
UseAAVExpressionMammalianPromoterEF1alpha, CBhAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_EGFP-P2A-HAUS6_IRES_Blast
Plasmid#182886PurposeTransfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (WT full length)DepositorInsertHAUS 6 (HAUS6 Human)
UseLentiviralTagsEGFPExpressionBacterial and MammalianPromoterEF1alpha coreAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZ854
Plasmid#208116PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-NeoDepositorInsertmNeonGreen-HPRT1-Neo
TagsT2AExpressionBacterial and MammalianPromoterEF1alphaAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT301 BxBi Lenti pEF-attB LSR GFP
Plasmid#184944PurposeLentiviral delivery of recombinase landing padDepositorInsertBxBi Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT306 Kp03 Lenti pEF-attB LSR GFP
Plasmid#184949PurposeLentiviral delivery of recombinase landing padDepositorInsertKp03 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDYT002
Plasmid#186550PurposeLineage tracing vector (with barcoded Target Sites and triple sgRNAsDepositorInsert14-bp random integration barcode and three target sites
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ853
Plasmid#208115PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSDDepositorInsertmNeonGreen-HPRT1-BSD
TagsT2AExpressionBacterial and MammalianPromoterEF1alphaAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJT302 Ec03 Lenti pEF-attB LSR GFP
Plasmid#184945PurposeLentiviral delivery of recombinase landing padDepositorInsertEc03 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT303 Ec04 Lenti pEF-attB LSR GFP
Plasmid#184946PurposeLentiviral delivery of recombinase landing padDepositorInsertEc04 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT304 Ec07 Lenti pEF-attB LSR GFP
Plasmid#184947PurposeLentiviral delivery of recombinase landing padDepositorInsertEc07 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX209-neo-HA-Ub-T2A-Tet3G
Plasmid#202436PurposeBicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)DepositorInsertUbiquitin
UseCRISPR and LentiviralExpressionMammalianPromoterEF1alphaAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ852
Plasmid#208114PurposemSwAP-In marker cassette 1 with mScarlet-HygTKDepositorInsertmScarlet-HygTK
TagsT2AExpressionBacterial and MammalianPromoterEF1alphaAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOX17-NLS-tdTomato-EPG
Plasmid#210466PurposeDonor plasmid for knock-in NLS-tdTomato into the human SOX17 locusDepositorInsertSOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
UseCRISPRAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV_PP1-mCitrine
Plasmid#172472PurposeMammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.DepositorInsertprolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
UseLentiviralTagsmCitrine and prolactin signal sequence (cleaved p…ExpressionMammalianPromoterEF1alphaAvailable SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-full-length-CHMP3-HA
Plasmid#154176Purposeexpresses mouse CHMP3 in mammalian cellsDepositorAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GP-TFIIB-2xStrep
Plasmid#213919PurposeSleeping beauty vector for expression of C-terminally strep-tagged TFIIBDepositorInsertTFIIB-2xStrep (GTF2B Human)
Tags2xStrepExpressionMammalianMutationCoding region changed for resistance to Dharmaco…PromoterEF-1EF1a/RPBSAAvailable SinceJune 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pGA1-EF1α-sfGFP150TAG-4xtRNA
Plasmid#251517PurposeExpression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cellsDepositorInsertsfGFP150TAG
TagsHis6 and V5ExpressionMammalianMutation150 TAG (amino acid 150 mutated to stop codon)PromoterEF1alphaAvailable SinceMarch 12, 2026AvailabilityAcademic Institutions and Nonprofits only