We narrowed to 22,439 results for: his

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CCM2(413-438)_pHisSUMO(K0)

Plasmid

#224065

Purpose

Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site

Depositor

Ben Goult

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

ccm2 (CCM2 Human)

Tags

6xHIS; SUMO(K0)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAM-AAV-hIsl2-EGFP

Plasmid

#153206

Purpose

Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-ccdB-SNAP

Plasmid

#166146

Purpose

Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10 and SNAP

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-Smt3-ccdB

Plasmid

#166147

Purpose

Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Type

Empty backbone

Tags

His10-Smt3

Expression

Bacterial

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-DDX5(kf)-SNAP

Plasmid

#166152

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Article

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Tags

His10 and SNAP

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-SUMO1-AVITAG-GG

Plasmid

#215808

Purpose

Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage non cleavable N-terminal avitag

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1

Plasmid

#205050

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L1

Tags

GRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2

Plasmid

#205051

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L2

Tags

GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3

Plasmid

#205052

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L3

Tags

GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R)-His6

Plasmid

#212778

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K63R)-His6

Plasmid

#212779

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo-yeGFP-His6

Plasmid

#212782

Purpose

C-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His6-CUbo-MCS

Plasmid

#212725

Purpose

Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His7-NUbo-MCS

Plasmid

#212724

Purpose

Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-NUbo-MCS-His6

Plasmid

#212723

Purpose

Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-His6-CUbo-GFP

Plasmid

#212720

Purpose

Bacterial expression of His6-CUbo-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

6xHis-CUbo

Expression

Bacterial

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a-His7-NUbo-GFP

Plasmid

#212719

Purpose

Bacterial expression of His7-NUbo-GFP

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

GFP

Tags

7xHis-NUbo

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-His6-TEV-Mms2

Plasmid

#212716

Purpose

Bacterial expression of His6-Mms2, His6-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Tags

6xHis-TEV

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mRhubarb713-HO1

Plasmid

#197254

Purpose

Expresses the protein of mRhubarb713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mRhubarb713

Expression

Bacterial

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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