We narrowed to 15,534 results for: LEA

1. pJS-BCD-Strep-SAVED-CHAT-sm

   Plasmid

   #214033

   Purpose

   Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   R456E, D459K, D476K, R479E

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJS-BCD-Strep-PCaspase

   Plasmid

   #214034

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218915

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 4330T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218913

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 5335T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218912

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-SARS-CoV2-NCAP/BlastR (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   2363G>C and 5335T>C

   Promoter

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET41-FeoB-OL4del

   Plasmid

   #216744

   Purpose

   Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Use

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET41-FeoB-OL5del

   Plasmid

   #216745

   Purpose

   Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 5 (OL5) by examining the variant lacking OL5 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Use

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Outer loop 5 (OL5) with the sequence of [ATFAA] i…

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET41-FeoB-OL2/4sub

   Plasmid

   #216746

   Purpose

   Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Use

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET41-FeoB-OL3/4sub

   Plasmid

   #216747

   Purpose

   Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   feoB (PA4358 P. aeruginosa (Bacteria))
   

   Use

   Tags

   8x His

   Expression

   Bacterial

   Mutation

   Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET41-FeoB-OL5/4sub

    Plasmid

    #216748

    Purpose

    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Article

    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

    Insert

    feoB (PA4358 P. aeruginosa (Bacteria))
    

    Use

    Tags

    8x His

    Expression

    Bacterial

    Mutation

    Outer loop 4 (OL4) with the sequence of [TLDGKPVQ…

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PCDF Bravo-PotH-OL1del

    Plasmid

    #216749

    Purpose

    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Article

    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

    Insert

    potH (potH E. coli (Bacteria))
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Outer loop 1 (OL1) with the sequence of [FKISLAEM…

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PCDF Bravo-PotH-OL2del

    Plasmid

    #216750

    Purpose

    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Article

    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

    Insert

    potH (potH E. coli (Bacteria))
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Outer loop 2 (OL2) with the sequence of [WMGILKNN…

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PCDF Bravo-PotH-OL3del

    Plasmid

    #216751

    Purpose

    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Article

    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

    Insert

    potH (potH E. coli (Bacteria))
    

    Use

    Tags

    10x His

    Expression

    Bacterial

    Mutation

    Outer loop 3 (OL3) with the sequence of [ELLGGPDS…

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET41-FeoB-OL1del

    Plasmid

    #216741

    Purpose

    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Article

    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

    Insert

    feoB (PA4358 P. aeruginosa (Bacteria))
    

    Use

    Tags

    8x His

    Expression

    Bacterial

    Mutation

    Outer loop 1 (OL1) with the sequence of [INIGGALQ…

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GRPR-DuET

    Plasmid

    #213306

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GRPR (GRPR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid

    #208966

    Purpose

    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-PDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA

    Plasmid

    #210502

    Purpose

    expresses LAUNCHERtvmv component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    PDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid

    #208973

    Purpose

    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-DIO-GRAB_CRF0.5

    Plasmid

    #208660

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-GRAB_CRF0.5

    Plasmid

    #208657

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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