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pet26-MBP-ELIC

Plasmid

#39239

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

Eriwinia chrysanthemi - pentameric ligand gated ion channel

Tags

His-10 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pJB94

Plasmid

#208752

Purpose

Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange

Depositor

Joseph Sorg

Article

Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

Insert

sacB (sacB )

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389-R3A

Plasmid

#8991

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, R3A (C-terminal RSPRR motif to ASPAA)

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-MCAK-C-7

Plasmid

#62785

Purpose

Localization: Microtubules/Kinesin, C1 Cloning Vector, Excitation:399, Emission:456

Depositor

Michael Davidson

Insert

MCAK (KIF2C Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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GST-Ub4

Plasmid

#171421

Purpose

For the purifcation of tetra linear ubiquitin

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

Ubiquitin

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJH124

Plasmid

#102951

Purpose

Expresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoter

Depositor

Peter Espenshade

Article

Hwang et al Biochem J. 2016 Jun 7. pii: BCJ20160106.

Insert

APEX2-NES

Tags

FLAG

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-F5A-E389-deltaCT

Plasmid

#8990

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

F5A, T389E, stop codon at amino acid 402

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pSR1037

Plasmid

#208736

Purpose

Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PSarA1

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1051

Plasmid

#208739

Purpose

Characterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PZwf

Use

Synthetic Biology

Expression

Bacterial

Promoter

PZwf

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSR1057

Plasmid

#208742

Purpose

Characterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PPgk

Use

Synthetic Biology

Expression

Bacterial

Promoter

PPgk

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pDM291-tetR-tup11Δ70

Plasmid

#41027

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetR-tup11Δ70

Expression

Yeast

Mutation

tup11 starts at aa70

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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TS_pLuxLac

Plasmid

#140426

Purpose

Toggle switch circuit responsive to AHL gradient.

Depositor

Yolanda Schaerli

Article

Barbier et al Mol Syst Biol. 2020 Jun;16(6):e9361. doi

Inserts

GFP
LacI
TetR
mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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mRFP1-pBAD

Plasmid

#54667

Purpose

Localization: Protein Expression Vector , Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

6xHis and mRFP1

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pRab-3::Cerulean-Venus::Lgg-1

Plasmid

#68045

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the neurons, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pRab-3::Cerulean-Venus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry

Plasmid

#239564

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA TrkC

Plasmid

#24089

Depositor

Moses Chao

Article

Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

Insert

TrkC (Ntrk3 Rat)

Expression

Mammalian

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL2807

Plasmid

#71203

Purpose

AD1 STAR Antisense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

AD1 Antisense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL2801

Plasmid

#71207

Purpose

AD1 Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

AD1 Sense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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