177,170 results
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Plasmid#67977PurposeCRISPR gRNA expression vector with an improved scaffold and puro/mCherry markersDepositorInsertU6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MI-Luciferase-IRES-mCherry
Plasmid#75020PurposeEncodes the expression of Luciferase and mCherryDepositorInsertsfirefly Luciferase
mCherry
UseLuciferase and RetroviralAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE-WT-RGVG-M6
Plasmid#70172PurposeExpresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNADepositorInsertRGVG-M6 T7 RNA polymerase
TagsHisExpressionBacterialPromoterT5/LacAvailable sinceDec. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFUGW-Pol2-ffLuc2-eGFP
Plasmid#71394PurposeLentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoterDepositorInsertPol2-ffLuc2-eGFP
UseLentiviralExpressionMammalianPromoterPol2 (mouse RNA polymerase II gene promtoer)Available sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
Tagsyeast mitochondrial targeting sequence of citrate…ExpressionYeastPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV:cTNT::Luciferase
Plasmid#69915PurposeUsed to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.DepositorInsertFirefly luciferase
UseAAVPromoterChicken cardiac troponin TAvailable sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-Cre-GFP
Plasmid#68544PurposeAAV expression of Cre-GFP fusionDepositorInsertCre
UseAAV and Cre/LoxTagsEGFPExpressionMammalianPromoterCMVAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1-TORCAR (LAMP1 Synthetic, Human)
TagsCerulean, Full-length LAMP1, and YPetExpressionMammalianPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV_Cas9_puro
Plasmid#65655PurposeRetroviral introduction of Cas9 into mammalian cell line.DepositorInsertCas9
UseCRISPR and RetroviralTags5 ' FLAG tagExpressionMammalianPromoterMSSV_LTRAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by