Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

176,531 results

Showing: 7461 - 7480 of 176531 results

  1. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid
    #162378
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8m
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    A25G F286Y
    Promoter
    Synapsin
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    25 Articles
    162378-plasmid-map-sequence-id-318373

    Enlarge

  8. pET15b_Bst-LF

    Plasmid
    #153313
    Purpose
    Bacterial expression of the Bst Large Fragment
    Depositor
    Drew Endy, Philippa Marrack
    Insert
    Bst Large Fragment
    Expression
    Bacterial
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    153313-plasmid-map-sequence-id-298756

    Enlarge

  12. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    139090-plasmid-map-sequence-id-273886

    Enlarge

  13. pENTR_L1-YFP-Turbo-L2

    Plasmid
    #127350
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, V5, and YFP
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127350-plasmid-map-sequence-id-246303

    Enlarge

  20. p426MET25_sfpHluorin (MRV55)

    Plasmid
    #115697
    Purpose
    superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)
    Depositor
    Eckhard Boles
    Publication
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    sfpHluorin
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    115697-plasmid-map-sequence-id-223644

    Enlarge

Showing: 7461 - 7480 of 176531 results