159,589 results

1. pNTI647 dCas9-Mxi1 TetR KanMX

   Plasmid

   #139474

   Purpose

   Integrates CRISPRi effector and tet-responsive regulator

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Inserts

   dCas9-Mxi1
   Tet Repressor
   

   Use

   CRISPR

   Tags

   Mxi1 and NLS

   Expression

   Yeast

   Mutation

   D10A, H840A

   Promoter

   pGPM1 and pTEF1

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159845

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PHB1

   Plasmid

   #212153

   Purpose

   For PHB production in E. coli (PT7-pMB1).

   Depositor

   Kang Zhou

   Insert

   poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-RIα-ICUE4

   Plasmid

   #181848

   Purpose

   ICUE4 cAMP sensor tethered to the PKA RIα subunit.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
   

   Use

   Tags

   ECFP, PKA RIα, and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. GW1-PercevalHR

   Plasmid

   #49082

   Purpose

   Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3349 pSFFV-neo Bcl-2

   Plasmid

   #8769

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pKLV2-U6gRNA5(NT)-PGKpuro2ABFP-W

   Plasmid

   #163169

   Purpose

   Lentiviral plasmid with non-targeting gRNA, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   Non-targeting
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. R777-E235 Hs.RASAL2

   Plasmid

   #70519

   Purpose

   Gateway ORF clone of human RASAL2 [NM_170692.2] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RASAL2 (RASAL2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGF-GAS4X

   Plasmid

   #225751

   Purpose

   This plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.

   Depositor

   Eun Hee Han

   Article

   Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

   Insert

   interferon gamma activating sequence (GAS) (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Use

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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