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pHAGE-BRAF-T599Ifs*13

Plasmid

#116132

Purpose

Lentiviral expression of BRAF T599Ifs*13

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

T599Ifs*13

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-T599Ffs*13

Plasmid

#116133

Purpose

Lentiviral expression of BRAF T599Ffs*13

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

T599Ffs*13

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-A762V

Plasmid

#116134

Purpose

Lentiviral expression of BRAF A762V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

A762V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-R401Q

Plasmid

#116112

Purpose

Lentiviral expression of ALK R401Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

R401Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ARAF-R188H

Plasmid

#116114

Purpose

Lentiviral expression of ARAF R188H

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ARAF (ARAF Human)

Use

Lentiviral

Mutation

R188H

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-B2M-L13Sfs*31

Plasmid

#116123

Purpose

Lentiviral expression of B2M L13Sfs*31

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

B2M (B2M Human)

Use

Lentiviral

Mutation

L13Sfs*31

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-B2M-M1L

Plasmid

#116124

Purpose

Lentiviral expression of B2M M1L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

B2M (B2M Human)

Use

Lentiviral

Mutation

M1L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-C990R

Plasmid

#116107

Purpose

Lentiviral expression of ALK C990R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

C990R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600L

Plasmid

#116207

Purpose

Lentiviral expression of BRAF V600L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600L

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600Ifs*13

Plasmid

#116198

Purpose

Lentiviral expression of BRAF V600Ifs*13

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600Ifs*13

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro DEST (w118-1) TSPAN9

Plasmid

#107502

Purpose

Expresses TSPAN9, puromycin resistant

Depositor

Alejandro Gutierrez

Article

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

TSPAN9 (TSPAN9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISSOarD1

Plasmid

#97067

Purpose

Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus Sso Ard1 protein

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV131

Plasmid

#97053

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 131

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV418/gp25

Plasmid

#97052

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 418/gp25

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 C3_26

Plasmid

#60306

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

OBFC2A enhancer (NABP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C3_34

Plasmid

#60313

Purpose

This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

PNMA2 enhancer (PNMA2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C5_12

Plasmid

#60319

Purpose

This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

Non active element (nearest TSS C18orf26)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.23 C5_14

Plasmid

#60321

Purpose

This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

Non active element (nearest TSS CCRN4L)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_7

Plasmid

#60256

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC30A8 enhancer (SLC30A8 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_15

Plasmid

#60262

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

FAM148A enhancer (C2CD4A Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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