4,183 results

1. pMKO.1 puro GFP shRNA

   Plasmid

   #10675

   Purpose

   Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   GFP shRNA
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ841

   Plasmid

   #39318

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   T7

   Available Since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. N-Myc-53BP1 WT pLPC-Puro

   Plasmid

   #19836

   Depositor

   Titia de Lange

   Article

   Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

   Insert

   p53 Binding Protein 1 (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVPT-tTR-KRAB

   Plasmid

   #11642

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. paavCAG-Jx-rev-post-mGRASP-2A-dTomato

   Plasmid

   #34913

   Purpose

   JxON-post

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   postsynaptic mGRASP
   

   Use

   AAV and Cre/Lox

   Promoter

   CAG

   Available Since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSKDuet01

   Plasmid

   #12172

   Depositor

   Hideo Iwai

   Article

   Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

   Insert

   NpuDnaE
   

   Tags

   His-GB1

   Expression

   Bacterial

   Available Since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-EF1a-BbTagBY

   Plasmid

   #45185

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Has Service

   AAV9

   Insert

   pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE
   

   Use

   AAV; Adeno-associated viral

   Expression

   Mammalian

   Promoter

   human EF1a

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPT-GFP

   Plasmid

   #12255

   Depositor

   Didier Trono

   Insert

   EGFP
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-mNeptune2-N

   Plasmid

   #41645

   Purpose

   Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)

   Depositor

   Michael Lin

   Article

   Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

   Insert

   mNeptune2-Dronpa145N
   

   Expression

   Mammalian

   Mutation

   K145N on Dronpa (allows control of photochromic F…

   Promoter

   CMV

   Available Since

   Dec. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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