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We narrowed to 10,337 results for: plasmids 101

Showing: 7501 - 7520 of 10337 results

  2. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

    Plasmid
    #111389
    Purpose
    AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)
    Depositor
    Adam Kepecs
    Article
    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi
    Inserts
    ChETA-mRuby2
    W3SL
    Use
    AAV
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    W3SL is built from a shortened WPRE, an upstream …
    Promoter
    hSynapsin
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    111389-plasmid-map-sequence-id-212217

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  3. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  5. pCKC775

    Plasmid
    #226626
    Purpose
    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    7xTCF-LEF and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226626-plasmid-map-sequence-id-449161

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  6. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, Chicken, S. pyogenes)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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  7. pCKC776

    Plasmid
    #226627
    Purpose
    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    Use
    Synthetic Biology
    Tags
    oxygen dependent degron (ODD)
    Expression
    Mammalian
    Mutation
    Promoter
    4xHRE_YB TATA and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226627-plasmid-map-sequence-id-449157

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  8. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

    Plasmid
    #120505
    Purpose
    Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    c-MYC deltaMBII (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of MYC Box II domain
    Promoter
    EF1a
    Available Since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    120505-plasmid-map-sequence-id-234918

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  11. pCSIIpuro-BTC-ScNeo

    Plasmid
    #209898
    Purpose
    To monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein
    Depositor
    Michiyuki Matsuda
    Article
    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi
    Insert
    BTC-ScNeo (BTC Human)
    Use
    Lentiviral
    Tags
    mNeonGreen and mScarlet
    Expression
    Mutation
    Promoter
    Available Since
    Nov. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    209898-plasmid-map-sequence-id-416606

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  12. pscAAV-Sox2-mStr

    Plasmid
    #135617
    Purpose
    Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus
    Depositor
    Lin He
    Article
    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e
    Insert
    P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    P2A-mStrawberry
    Expression
    Mutation
    Promoter
    Available Since
    May 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135617-plasmid-map-sequence-id-263111

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  14. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

    Plasmid
    #105000
    Purpose
    Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Article
    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.
    Insert
    Renilla luciferase
    Use
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TetCMV
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105000-plasmid-map-sequence-id-203163

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  17. INS-GFP-CD19-EPT

    Plasmid
    #210469
    Purpose
    Donor plasmid for knock-in GFP-CD19 into the human INS locus
    Depositor
    Kai Wang
    Article
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    210469-plasmid-map-sequence-id-419052

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Showing: 7501 - 7520 of 10337 results