4,183 results

1. pLVET-tTR-KRAB

   Plasmid

   #11644

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hEF-1alpha, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. PERK 1: PERK.WT.9E10.pCDNA

   Plasmid

   #21814

   Depositor

   David Ron

   Article

   Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

   Insert

   PERK (Eif2ak3 Mouse)
   

   Tags

   Myc (9E10)

   Expression

   Mammalian

   Available Since

   March 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIG-Aalpha WT

   Plasmid

   #10884

   Depositor

   William Hahn

   Article

   Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

   Insert

   PP2A Aalpha (PPP2R1A Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetO-FUW-oct4

   Plasmid

   #20323

   Depositor

   Rudolf Jaenisch

   Article

   Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

   Insert

   oct4 (Pou5f1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ350 - MCS(ttTi5605, II)

   Plasmid

   #34866

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

   Insert

   ttTi5605 targeting
   

   Use

   Worm targeting

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQLinkH

   Plasmid

   #13667

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   His, TEV, and attB1

   Expression

   Bacterial

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6-DEST PINK1-V5 KD

   Plasmid

   #13319

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Kinase dead: K219A/D362A/D384A

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-Foxo4

   Plasmid

   #17549

   Depositor

   Domenico Accili

   Insert

   FoxO4 (FOXO4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 puro shRNA E-cadherin

   Plasmid

   #18801

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   E-cadherin shRNA (CDH1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 22, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-DNMT3A-PuroR_v2

    Plasmid

    #74407

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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