We narrowed to 16,821 results for: IGH@

1. p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)

   Plasmid

   #112803

   Purpose

   Expression of OCTN2 mutant

   Depositor

   Katarzyna Nalęcz

   Article

   Jurkiewicz et al Biochim Biophys Acta. 2017 May;1864(5):7

   Insert

   OCTN2/SLC22A5 (Slc22a5 Rat)
   

   Use

   Tags

   3 x Flag

   Expression

   Mammalian

   Mutation

   amino acids 554_557 deleted/PDZ

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_DEPTOR_DEP

   Plasmid

   #109882

   Purpose

   Protein expression and purification of DEPTOR_DEP

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   DEPTOR_DEP (DEPTOR Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTG-mTDGa.0

   Plasmid

   #81048

   Purpose

   bacterial expression of murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-30a(+)-N-SH2

   Plasmid

   #111274

   Purpose

   express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
   

   Use

   Tags

   HHHHHHENLYFQG

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGS_N_Strep

    Plasmid

    #101651

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, Twin-Strep tag
    

    Use

    Tags

    Twin-Strep tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGS_C_Strep

    Plasmid

    #101652

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, Twin-Strep tag
    

    Use

    Tags

    Twin-Strep tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMflCT-o4

    Plasmid

    #101313

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    cat resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMflT-o3

    Plasmid

    #101311

    Purpose

    Contains: oriC3 fragment of M. florum L1 replication origin (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA), colE1 rep origin, recoded tetM resistance gene, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LLhT-pHG165a V52A

    Plasmid

    #90050

    Purpose

    Constitutive expression of a chimeric LacI:TreR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhT/V52A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V52A

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLhA-pHG165a Q55A

    Plasmid

    #90052

    Purpose

    Constitutive expression of a chimeric LacI:AscG transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhA/Q55A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q55A

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LLhC-pHG165a Q55I

    Plasmid

    #90053

    Purpose

    Constitutive expression of a chimeric LacI:CytR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

    Insert

    LLhC/Q55I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q55I

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RGD-I27-CC

    Plasmid

    #85393

    Purpose

    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling

    Depositor

    Khalid Salaita

    Article

    Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

    Insert

    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL2-MCT2Prom2.5

    Plasmid

    #87351

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 2.5 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    2.5 kbp proximal promoter of human MCT2 (nested d…

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL2-MCT2Prom1.1

    Plasmid

    #87352

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 1.1 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    1.1 kbp proximal promoter of human MCT2 (nested d…

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL2-MCT2Prom3.2

    Plasmid

    #87349

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 3.2 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    3.2 kbp proximal promoter of MCT2 (nested deletio…

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCIneoRL-BCL2 3'UTR-mir200c-mut

    Plasmid

    #84598

    Purpose

    Renilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    BCL2 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on BCL2 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pCIneoRL-TOB1 3'UTR-mir200c-mut

    Plasmid

    #84590

    Purpose

    Renilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    TOB1 3'UTR (UTS2R Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on TOB1 3'…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pCIneoRL-RanBP93'UTR-mir200c-mut

    Plasmid

    #84592

    Purpose

    Renilla Luciferase reporter assay for RANBP9 3'UTR with mutations on miR-200c binding site

    Depositor

    Yanan Yang

    Article

    Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

    Insert

    RANBP9 3'UTR (RANBP9 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mutation

    Mutation on miR-200c binding site on RANBP9 3…

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pNIA-CEN-FLAG-LINXa4-Bre1

    Plasmid

    #73617

    Purpose

    Control of the E3 ligase Bre1 with LINXa in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa4-Bre1dNLS
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHL836

    Plasmid

    #62827

    Purpose

    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    gfp
    ipex leader
    hfq leader
    

    Use

    Synthetic Biology

    Tags

    ompC leader and st7 RBS

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pHL1954

    Plasmid

    #62831

    Purpose

    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::RBS (st7) hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    M8::ryhB leader
    hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::sodB leader and st7 RBS

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pHL1969

    Plasmid

    #62832

    Purpose

    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    M8::ryhB leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::sodB leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pHL2107

    Plasmid

    #62841

    Purpose

    Lim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    m8::micC leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::ompC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

29. mCardinal-Cx26-7

    Plasmid

    #56156

    Purpose

    Localization: Gap Junctions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Cx26
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. mCardinal-CAF1-C-10

    Plasmid

    #56153

    Purpose

    Localization: Chromatin Assembly Factor 1, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    CAF1
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits