Addgene

We narrowed to 16,452 results for: EMB

Showing: 7541 - 7560 of 16452 results

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pFA6a-kanMX6-P41nmt1-mEGFP

Plasmid

#105140

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P41nmt1-mEGFP

Use

Tags

41nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2361

Plasmid

#36774

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX8-TALEN_Right (Cbx8 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TOPO CMV

Plasmid

#68454

Purpose

TOPO construct expressing CMV for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Cytomegalovirus Immediate-Early Promoter

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187

Plasmid

#139309

Purpose

N-terminal half of split PI-PLC targeted to mitochondria

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R365

Plasmid

#138235

Purpose

Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Mesp-1916/-1>nls::Cas9::nls

Plasmid

#59988

Purpose

Mesp driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Mesp -1916/-1

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pX28

Plasmid

#46850

Purpose

TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB, RFP and PGK-puro

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Chronos(M140T)-mRuby2-ST

Plasmid

#109129

Purpose

Modified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

Chronos(M140T)-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2412

Plasmid

#36813

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BCL6-TALEN_Left (Bcl6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2401

Plasmid

#36804

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC6-TALEN_Right (Hdac6 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pF(UG) hSyn SYP-SELF

Plasmid

#158770

Purpose

Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptophysin 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2360

Plasmid

#36773

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CBX8-TALEN_Left (Cbx8 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
p068 pCS mTRPS1 (M)

Plasmid

#11040

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 mut (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Two Cys residues at positions 917 and 920 in the …

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-171-1792

Plasmid

#41661

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 171-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
3087 pBS Bcl-2-Ig

Plasmid

#8809

Depositor

Stanley Korsmeyer

Article

Seto et al EMBO J 1988 Jan;7(1):123-31.

Insert

Bcl-2-Ig (BCL2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-831-1281

Plasmid

#40962

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830 and 1282-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1282-1792

Plasmid

#40960

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1281

Promoter

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
pUC19-P70a-Top-T~Bottom-T

Plasmid

#87315

Purpose

Control plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system (promoter control)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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UAS-akkordion dNhe

Plasmid

#41976

Depositor

Norbert Perrimon

Article

Hacker et al Genes Dev. 1998 Jan 15;12(2):274-84.

Insert

DRhoGEF2 (RhoGEF2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only
S3_004_026_MV1

Plasmid

#134416

Purpose

pYTKMV1-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Article

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Use

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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