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pRS315e_pGal-PmCDA1

Plasmid

#79612

Purpose

Expresses PmCDA1 in yeast cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

PmCDA1

Expression

Yeast

Promoter

pGal1

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCrAb-km

Plasmid

#214199

Purpose

Expresses crRNA for genome editing in Acinetobacter baumannii

Depositor

Yu Wang

Publication

Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

Insert

crRNA-HDV

Use

CRISPR

Promoter

J23119

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10-mNeonGreen-GW

Plasmid

#209352

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.

Depositor

Lucia Strader

Publication

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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ROS lux sensor

Plasmid

#199791

Purpose

Synthetic gene circuit

Depositor

Timothy Lu

Publication

Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

Insert

ROS lux Sensor

Use

Synthetic Biology

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-A4DARPin

Plasmid

#198288

Purpose

Produces PVCs retargeted with anti-lysozyme DARPin A4

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-A4DARPin

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBW-507

Plasmid

#195469

Purpose

Lentiviral Integratable Constitutive CD19CAR

Depositor

Ahmad Khalil

Publication

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV-CD19CAR-mCherry

Use

Lentiviral

Expression

Mammalian

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shControl

Plasmid

#180386

Purpose

Producing AAV that encodes negative control shRNA with miR-E backbone

Depositor

Huda Zoghbi

Publication

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shControl

Use

AAV and RNAi

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJIT163-eAFID-3

Plasmid

#167360

Purpose

For inducing predictable deletions in protoplasts and wheat plants

Depositor

Caixia Gao

Publication

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3Bctd-Cas9-UDG-T2A-AP lyase

Expression

Plant

Mutation

WT

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-mChe

Plasmid

#162753

Purpose

Retroviral (MSCV) expression of mCherry fluorescent protein

Depositor

Maki Nakayama

Publication

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCri_L706-antiSH2-OptoMB

Plasmid

#159935

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Publication

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Expression

Bacterial

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(0)_T2AGAL4

Plasmid

#125211

Purpose

Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Publication

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKAd5f11pABR-EPG

Plasmid

#122699

Purpose

It can be used to construct a recombinant virus carrying other transgene or an adenovector with modified fiber.

Depositor

Zhuo-Zhuang Lu

Publication

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Insert

GFP

Use

Adenoviral

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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DRH323: pAAV_hSyn-QuasAr2

Plasmid

#107705

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Publication

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr2

Use

AAV

Expression

Mammalian

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKEW303-AcrVA5

Plasmid

#115657

Purpose

Expresses AcrVA5 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCZ829

Plasmid

#110137

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Publication

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito-GCaMP5

Tags

mito::GCaMP5

Expression

Worm

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_B2519

Plasmid

#91076

Purpose

Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU3

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDH18

Plasmid

#83803

Purpose

YFP-CFP positive FRET control; his selection

Depositor

Eric Muller

Publication

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP-CFP

Expression

Yeast

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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CH00X

Plasmid

#73517

Purpose

TagRFP-T_PH pCFJ150 - contains C. elegans codon optimized TagRFP-T

Depositor

Bob Goldstein

Publication

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::TagRFP-T::PH::tbb-2

Tags

TagRFP-T

Promoter

mes-5

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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JH04

Plasmid

#73513

Purpose

mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

Depositor

Bob Goldstein

Publication

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mRuby2::PH::tbb-2

Tags

mRuby2

Promoter

mex-5

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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mTagRFP-CytERM-N-17

Plasmid

#57985

Purpose

Localization: Endoplasmic Reticulum, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Insert

CytERM

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits