We narrowed to 10,906 results for: 158
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Plasmid#184973PurposeTest effect of extending a1/a2 on ADE2 editing rates in yeastDepositorInsertEco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
ExpressionYeastMutationADE2 donor P272stop, a1/a2 length extended to 27 …PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
nPPARg429-gs10-NLS-VPR
Plasmid#188266PurposePlasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnPPARg429 (PPARG Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.407
Plasmid#187204PurposeExpresses retron Eco1 ncRNA v32, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v32
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.408
Plasmid#187205PurposeExpresses retron Eco1 ncRNA v35, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA v35
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRExpressionMammalianAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV5endoNA2
Plasmid#174729PurposeExpresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertV5 epitope tag
TagsHistagExpressionBacterialPromoterT5Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-aACry::EGFP-zebrafish Plaat1 (WT)
Plasmid#168772PurposeExpress EGFP-zebrafish Plaat1 (WT) in zebrafish lensDepositorAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_4E2H.210
Plasmid#155212PurposeExpresses 4E2H.210 de novo protein in E.coliDepositorInsert4E2H.210
Tags6x His TagExpressionBacterialAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW927
Plasmid#115961PurposeORF insert for replicon MoCloDepositorInsertLvl0 ORF TetR-2A-mKate-PEST GG
UseSynthetic BiologyAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW099
Plasmid#115942PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/14 SGP 2x K-turn
UseSynthetic BiologyAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND4_L17_S24_S11_S19_S13
Plasmid#132732PurposeT7 RNAP-driven expression of a 4-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 24, 11, 19, and 13DepositorInsert3WJ_NAND4_L17_S24_S11_S19_S13
ExpressionBacterialAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG_Ptet*_ToeRep_N01_switch
Plasmid#132737PurposeInducible expression of first-generation toehold repressor index 1 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_ToeRep_N01_switch
ExpressionBacterialAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_Ptet*_3WJrep_N19_switch
Plasmid#132739PurposeInducible expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter via the Ptet* promoterDepositorInsertPtet*_3WJrep_N19_switch
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_NAND3_L11_S11_S13_S19
Plasmid#132731PurposeT7 RNAP-driven expression of a 3-input NAND gate RNA with a GFP-ASV reporter that binds to 3WJ repressor trigger indices 11, 13, and 19DepositorInsert3WJ_NAND3_L11_S11_S13_S19
ExpressionBacterialAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD286
Plasmid#125074PurposeExpression of SO_4622 under PtacDepositorUseSynthetic BiologyExpressionBacterialMutationS286L- Please see depositor comments belowAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD276
Plasmid#125069PurposeExpression of SO_0621 under PtacDepositorUseSynthetic BiologyExpressionBacterialMutationS286L- Please see depositor comments belowAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD250.2
Plasmid#125077PurposeExpression of torR under PLtetO-1, output promoter PtorCAD129DepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL251 GG sgRNA
Plasmid#126436PurposeTo target a protospacer with GG at the cut site.DepositorInsertspacer against human genome with GG at cut site
ExpressionMammalianPromoterhuman U6Available SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only