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Plasmid#59014PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR1.1
Plasmid#59011PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR1.1 genetically encoded Zn(II) sensor
UseTagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-mCherry
Plasmid#45147DepositorInsertLAMP1-mCherry (LAMP1 Human, Synthetic)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-TagRFP-T-ps
Plasmid#178656PurposeExpression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cellsDepositorInsertTagRFP-T with palmitoylation signal at N-terminus
UseLentiviralTagsPalmitoylation signalExpressionMutationPromoterUbcAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49,50,83,103A
Plasmid#58421PurposeExpresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, 83, and 103 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-10xHis-ecRNHI*
Plasmid#219523Purposebacterial expression of cysteine-free variant of the E. coli ribonuclease HI geneDepositorInsertcysteine-free variant of the E. coli ribonuclease HI gene
UseTagsN terminal 10xHis TagExpressionBacterialMutationCysteine free variantPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-Let7d-5p
Plasmid#117055PurposeOverexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expressionDepositorInsertLet-7d-5p - OFP
UseLentiviralTagsExpressionMutationPromoterSFFV promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
env8-AD-5'antiPNA-U1
Plasmid#233011PurposeU1 snRNA tagged with 1 copy of Riboglow (truncated variant A with 5' antiPNA motif)DepositorInsertU1 (RNU1-1 Human)
UseTagsExpressionMammalianMutation1 copy of Riboglow tag (truncated variant A with …PromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pLentiDP1.0
Plasmid#171094Purposelentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2A-sgRNA
Plasmid#182551PurposeCas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2A-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2B-sgRNA
Plasmid#182552PurposeCas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2B-sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterCbhAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDP1.2
Plasmid#171092PurposeExpresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)DepositorInsertAAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
UseSynthetic BiologyTagsEmerald, GemininExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AT-U1
Plasmid#112059PurposeU1 snRNA tagged with 1 copy of Riboglow (truncated variant A)DepositorInsertU1 (RNU1-1 Human)
UseTagsExpressionMammalianMutation1 copy of Riboglow tag (truncated variant A) near…PromoterAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.3
Plasmid#171096Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, cdt1, miniAID, mCherry2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.2
Plasmid#171095Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, Geminin, miniAID, mCherry2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-miR-142
Plasmid#117056PurposeOverexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expressionDepositorInsertmiR-142-3p - OFP (Mir142 Mouse)
UseLentiviralTagsExpressionMutationPromoterSFFV promoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)4x
Plasmid#112058PurposeACTB mRNA tagged with 4 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation4 copies of Riboglow tag after stop codonPromoterCMVAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)1x
Plasmid#112055PurposeACTB mRNA tagged with 1 copy of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
UseTagsRiboglow RNA tagExpressionMammalianMutation1 copy of Riboglow tag (variant A) after stop cod…PromoterCMVAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only