We narrowed to 5,868 results for: nls

1. mKikGR-PCNA-19-SV40NLS-4

   Plasmid

   #57328

   Purpose

   Localization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mKikGR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. 1xNLS-pMJ915v2-sfGFP

   Plasmid

   #88919

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mKO2-PCNA-19-SV40NLS-4

   Plasmid

   #57890

   Purpose

   Localization: Nucleus, Excitation: 551, Emission: 565

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2xNLS-pMJ915v2-sfGFP

   Plasmid

   #88920

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-BE3-FNLS

   Plasmid

   #110836

   Purpose

   CMV expression vector for BE3-FNLS construct (not codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEBG-TFII-deltaNLS1

   Plasmid

   #22153

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Use

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   deletion of amino acids 297-304

   Promoter

   Available since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mEmerald-PCNA-19-SV40NLS-4

   Plasmid

   #54223

   Purpose

   Localization: Nucleus, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   3xSV40NLS and mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. mOrange2-PCNA-19-SV40NLS-4

   Plasmid

   #57971

   Purpose

   Localization: Nucleus, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pTcTurboID-GW_GFP-NLS

   Plasmid

   #234716

   Purpose

   Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.

   Depositor

   Esteban Serra

   Insert

   GFP-3xNLS
   

   Use

   Trypanosomatid expression

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. TK4-NLS-EGFP

    Plasmid

    #239046

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2 and c-myc-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP-TDP-43 (PY-NLS)

    Plasmid

    #235490

    Purpose

    Expression of chimeric human TDP-43-EGFP bearing a PY-nuclear localization sequence (NLS) from HNRNPA1 in place of the native NLS sequence

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Replaced native NLS with PY-NLS from HNRNPA

    Promoter

    Available since

    May 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pEGFP-TDP-43 (NLSmut)

    Plasmid

    #235489

    Purpose

    Expression of human TDP-43-EGFP with mutated nuclear localization sequence

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, K95A, K97A, R98A

    Promoter

    Available since

    May 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pENTR-nlsABACUS2-400n

    Plasmid

    #203726

    Purpose

    Gateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-polyQ23-NLS

    Plasmid

    #231891

    Purpose

    Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q23-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-NLS-mCherry

    Plasmid

    #231890

    Purpose

    mCherry-tagged nuclear rupture marker

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    mCherry-SV40 NLS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UAST-uMCas12a+nls2x

    Plasmid

    #230909

    Purpose

    inducible expression of Cas12a+ with Gal4.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV_dCas9-AsLOV2x3-NLSx3

    Plasmid

    #231567

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    dCas9-AsLOV2x3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. T2A 3xNLS GFP

    Plasmid

    #227721

    Purpose

    Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

    Depositor

    Oliver Hobert, Haosheng Sun

    Article

    Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

    Insert

    T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAC3-C-GFP-NLS

    Plasmid

    #222604

    Purpose

    Expression of MAC3-tagged GFP-NLS (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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