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pcDNA3.1-Hyg-mEGFP

Plasmid

#191847

Purpose

Expresses the monomeric form of EGFP

Depositor

Bertrand Collet

Article

Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

Insert

mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TORCAR-NES

Plasmid

#175015

Purpose

Cytosol-targeted TORCAR.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

TORCAR-NES

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA)

Plasmid

#192442

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A) (TSC2 Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PCDNA3-N3FLAG-MYB-FL

Plasmid

#105609

Purpose

transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Tags

3*FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-AktAR2

Plasmid

#175013

Purpose

Plasma membrane-targeted AktAR2.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Use

Tags

N-terminal targeting sequence from Lyn kinase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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hJAM-A deltaCT pcDNA3.1

Plasmid

#70074

Purpose

Expresses human junctional adhesion molecule-A amino acids 1-260 in mammalian cells

Depositor

Terence Dermody

Article

Danthi et al J Virol. 2006 Feb;80(3):1261-70.

Insert

junctional adhesion molecule-A (F11R Human)

Use

Tags

Expression

Mammalian

Mutation

deleted C-terminal cytoplasmic tail (amino acids …

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-PPARgamma2 2KR

Plasmid

#78772

Purpose

To overexpress PPARgamma2 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

Insert

PPAR_2 (Ppargc1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

K184A, K185A

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-FLAG-Rgs14

Plasmid

#236396

Purpose

Expresses FLAG-Rgs14 in mammalian cells

Depositor

John Hepler

Article

Shu et al Cell Signal. 2007 Jan;19(1):163-76. doi:

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGCC2-FLAG

Plasmid

#214166

Purpose

Transient expression of GCC2

Depositor

Nan Yan

Article

Tu et al Nat Commun. 2022 Nov 15;13(1):6977. doi:

Insert

GCC2 (Gcc2 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pcDNA3

Plasmid

#206053

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585_Y167F

Plasmid

#174392

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Use

Tags

Flag

Expression

Mammalian

Mutation

Tyr 167 changed to Phe

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.S61P

Plasmid

#105048

Purpose

Overexpression of APOBEC3C S61P mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

serine 61 to proline

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.D99E

Plasmid

#105049

Purpose

Overexpression of APOBEC3C D99E mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Aspartate 99 to glutamate

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.DS-EP

Plasmid

#105050

Purpose

Overexpression of APOBEC3C DS-EP mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

S61P, D99E

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human A3C.C97S

Plasmid

#105051

Purpose

Overexpression of APOBEC3C C97S mutant in mammalian cell lines

Depositor

Carsten Münk

Article

Jaguva Vasudevan et al J Mol Biol. 2017 Apr 21;429(8):1171-1191

Insert

APOBEC3C (A3C) (APOBEC3C Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

cysteine 97 to serine

Promoter

CMV

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mCherry Gamma 3

Plasmid

#65234

Purpose

To study the role of the differential rates of Gβγ translocation in modulating signaling dynamics in a cell

Depositor

Narasimhan Gautam

Article

Giri et al Biophys J. 2014 Jul 1;107(1):242-54. doi

Inserts

mcherry
Gng3 (Gng3 Mouse)

Use

Tags

6-His tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
HcCCR_mCherry_pcDNA3.1

Plasmid

#201687

Purpose

expresses HcCCR_mCherry fusion in mammalian cells

Depositor

John Spudich

Article

Govorunova et al mBio. 2022 Dec 20;13(6):e0303922. doi: 1

Insert

HcCCR

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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CovKCR1_mCherry_pcDNA3.1

Plasmid

#201688

Purpose

expresses CovKCR1_mCherry fusion in mammalian cells

Depositor

John Spudich

Article

Govorunova et al mBio. 2022 Dec 20;13(6):e0303922. doi: 1

Insert

CovKCR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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XE212 RGS9-pcDNA3.1

Plasmid

#16676

Depositor

Randall Moon

Insert

RGS9 (Rgs9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-T7-ERK1

Plasmid

#14440

Depositor

John Blenis

Insert

ERK1 (Mapk3 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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