2,294 results

1. Rescue Septin 11 mRNA

   Plasmid

   #122022

   Purpose

   Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Expression

   Mammalian

   Mutation

   Silent mutations are in 5 consecutive codons (AUU…

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV302

   Plasmid

   #119943

   Purpose

   Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

   Insert

   N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

   Plasmid

   #123614

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A)-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSW GAP43-jRGECO1a P2A Syp-Halo

   Plasmid

   #185565

   Purpose

   Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

   Insert

   GAP43-jRGECO1a P2A Syp-Halo
   

   Use

   Lentiviral

   Tags

   HaloTag

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX-PGK-LAMP1-EYFP

   Plasmid

   #164215

   Purpose

   pLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   EYFP

   Promoter

   PGK

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSV2 neuT

   Plasmid

   #10919

   Depositor

   Bob Weinberg

   Article

   Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

   Insert

   neuT (Erbb2 Rat)
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-CMV-DIO-Magneto2.0-sNRPpA

   Plasmid

   #74307

   Purpose

   Cre-dependent expression of Magneto2.0 in mammalian cells

   Depositor

   Ali Guler

   Article

   Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

   Insert

   Magneto2.0 (FTL, FTH1, Trpv4 Human, Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   TRPV4: delta 760-871

   Promoter

   CMV (after Cre recombination)

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT2 Beta1b GFP

   Plasmid

   #89893

   Purpose

   Mammalian expression of Beta1b GFP

   Depositor

   Annette Dolphin

   Article

   Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

   Insert

   Cacnb1 (Cacnb1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   GFP sequence inserted just before the Stop codon

   Promoter

   adenovirus major late promoter

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFlc245

   Plasmid

   #101021

   Purpose

   fluorescent, functional, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   GluR 1 (Gria1 Rat)
   

   Expression

   Mammalian

   Mutation

   fluorescent protein inserted into subunit

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. T alpha 1p-DsRed2

    Plasmid

    #17707

    Depositor

    Nicholas Gaiano

    Article

    Mizutani et al Nature. 2007 Sep 20. 449(7160):351-5.

    Insert

    T alpha 1p (Tuba1a Rat)
    

    Tags

    DsRed2

    Expression

    Mammalian

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pORANGE GFP-Pclo KI

    Plasmid

    #139657

    Purpose

    Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CACNA2D1-TwinStrepII

    Plasmid

    #83192

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    CACNA2D1 (Cacna2d1 Rat)
    

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. RIP-Timer (DM#285)

    Plasmid

    #15109

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    insulin II promoter (Ins2 Rat)
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK284 (Stag-3FLAG-Hygro)

    Plasmid

    #72800

    Purpose

    C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    Stag-3FLAG-Hygro
    

    Use

    CRISPR

    Tags

    Stag-3FLAG

    Expression

    Mammalian

    Available Since

    March 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_EGFP-RandE3

    Plasmid

    #79870

    Purpose

    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

    Depositor

    Don Arnold

    Article

    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

    Insert

    GFP-RandE3
    

    Use

    Synthetic Biology

    Tags

    GFP and HA

    Expression

    Mammalian

    Promoter

    Beta-actin

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT2 Beta1b mCherry

    Plasmid

    #89892

    Purpose

    Mammalian expression of Beta1b mCherry

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry sequence inserted just before the Stop co…

    Promoter

    adenovirus major late promoter

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV312.3

    Plasmid

    #119944

    Purpose

    Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

    Insert

    C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-MyosinVb-Ctail

    Plasmid

    #110170

    Purpose

    Dominant negative effect by competing with MyosinVb C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Vb (Myo5b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 1262-1670

    Promoter

    CMV

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-GCaMP3fast

    Plasmid

    #74422

    Purpose

    Express fluorescent calcium indicator GCaMP3fast in mammalian cells

    Depositor

    Katalin Torok

    Article

    Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

    Insert

    GCaMP3fast
    

    Expression

    Mammalian

    Mutation

    W4Y D356A

    Promoter

    CMV

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. DHFR-mNeon-GluA1

    Plasmid

    #173009

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-mNeon-GluA1
    

    Tags

    DHFR and mNeon

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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