Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,333 results for: ef1a

Showing: 1481 - 1520 of 7333 results

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EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH

Plasmid

#235266

Purpose

ComMAND EGFP-FMRP open-loop circuit

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH

Plasmid

#235267

Purpose

ComMAND EGFP-FMRP closed-loop circuit

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

Plasmid

#194232

Purpose

Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB3-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-HexaPro_Foldon-PDGFR-T2A-mCherry (pZYW079)

Plasmid

#194234

Purpose

Lentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-Spike ECD-PDGFR TMD-T2A-mCherry (S Synthetic)

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552 EF1a DIO Chronos GFP_Vgat gRNA

Plasmid

#215277

Purpose

Expresses Vgat gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat gRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX552 EF1a DIO Chronos GFP_VgatTTT gRNA

Plasmid

#215278

Purpose

Expresses Vgat Control gRNA in a Cre dependent Chronos GFP vector

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Vgat control gRNA

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p931-SWITCH-OVER insert: EF1a-Blasti

Plasmid

#217890

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP

Plasmid

#216476

Purpose

lentiviral dual expression of dsRed and tagBFP

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Lentiviral

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep

Plasmid

#213542

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 Orf3a-2xStrep (ORF3a Severe acute respiratory syndrome coronavirus 2, Human)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep

Plasmid

#213543

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 protE-2xStrep (E Severe acute respiratory syndrome coronavirus 2, Human)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-10_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211670

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-10 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-5_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211665

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-5 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-6_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211666

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-6 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-7_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211667

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-7 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgRPA1-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211659

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgRPA1-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-4_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211664

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-4 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-21_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211671

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-21 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-22_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211672

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-22 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgOR2B6-1_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211655

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgOR2B6-1 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgOR2B6-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211656

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgOR2B6-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgRPA1-3_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211660

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgRPA1-3 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211662

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-8_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211668

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-8 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-9_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211669

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-9 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-42_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211682

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-42 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgNT-1_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211652

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-1 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-43_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211683

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-43 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF820-sgCIDE-1_U6-sgRNA-EF1a-mCherry2

Plasmid

#211649

Purpose

U6-sgRNA-EF1a-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

sgCIDE-1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF820-sgCIDE-2_U6-sgRNA-EF1a-mCherry2

Plasmid

#211650

Purpose

U6-sgRNA-EF1a-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

sgCIDE-2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-35_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211679

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-35 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-3_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211663

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-3 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-44_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211684

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-44 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-46_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211686

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-46 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgNT-2_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211653

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-2 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-31_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211675

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-31 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgNT-3_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211654

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgNT-3 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-23_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211673

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-23 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCF821-sgCIDE-45_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211685

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and sgCIDE-45 guide RNA vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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