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pENTR-Creb5(T59/T61D)(HA)

Plasmid

#195026

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Gateway cloning

Tags

3xHA

Expression

Mutation

T59/T61D

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Creb5(HA)

Plasmid

#195024

Purpose

HA-tagged Creb5 in pLenti CMV Puro DEST (w118-1) vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(T59/T61A)(HA)

Plasmid

#195027

Purpose

HA-tagged Creb5(T59/T61A) in pInducer20 lentivirus destination vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with T59/T61A mutation) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T59/T61A

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer20-iCreb5(T59/T61D)(HA)

Plasmid

#195028

Purpose

HA-tagged Creb5(T59/T61D) in pInducer20 lentivirus destination vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T59/T61D

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pTE3314

Plasmid

#107530

Purpose

Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(RVR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N607R, K613V, N617R

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3319

Plasmid

#107533

Purpose

Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(RR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N576R, K637R

Promoter

CMV

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3316

Plasmid

#107532

Purpose

Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(RR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N607R, K671R

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3317

Plasmid

#107531

Purpose

Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(RVR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N576R, K582V, N586R

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-Cre

Plasmid

#121675

Purpose

Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

Depositor

Esteban Engel, Alexander Nectow

Article

Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

Has Service

AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

Insert

NLS-CRE-HA

Use

AAV

Tags

3xHA and SV40-NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-opEBNA2-IRES-GFP

Plasmid

#220354

Purpose

Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

Depositor

Klaus Rajewsky

Article

Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

Insert

opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)

Use

Retroviral

Tags

3 x HA tag

Expression

Mammalian

Mutation

codon-optimised for expression in mammalian cells

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1-GFP (A317-318; A341)

Plasmid

#204198

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Use

Tags

3xHA, eGFP and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

Plasmid

#173029

Purpose

For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

Depositor

Mohamed Fareh

Article

Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

Insert

pspCas13b

Use

CRISPR and Lentiviral

Tags

3xFLAG, BFP, HIV NES, and T2A

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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6-His-MBP-TEV-FnCpf1

Plasmid

#90094

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 (humanized)

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-scFVtet1_bGHpA

Plasmid

#177351

Purpose

AAV expression of scFV-fused catalytic domain of TET1 for targeted DNA methylation editing

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

AAV

Tags

myc and scFV

Expression

Mammalian

Mutation

N terminal domain of human Tet1

Promoter

CMV promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Dual-CRISPR-Cas9-U6-H1 (pBP48)

Plasmid

#218932

Purpose

The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

Depositor

Baoxu Pang

Article

Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

Type

Empty backbone

Use

CRISPR

Tags

2A-Puro and 3XHA

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTE4497

Plasmid

#80339

Purpose

mammalian expression FnCpf1 nuclease and Fn crRNA

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
FnCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTE4889

Plasmid

#88905

Purpose

Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
hFnCpf1(D917A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

Plasmid

#121841

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSH-Csy4-T2A-SaRFN

Plasmid

#85755

Purpose

Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Article

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Insert

Csy4-T2A-FokI-SadCas9

Use

CRISPR

Tags

3xHA tag, Csy4, and FokI fusion via 36 amino acid…

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CAG

Available since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-Mettl3

Plasmid

#196262

Purpose

In vitro transcription/translation of HA-tagged wild-type Mettl3

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 (Mettl3 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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